| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASQ |
| Uniprot No | P31415 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-396aa |
| 氨基酸序列 | QEGLDF PEYDGVDRVI NVNAKNYKNV FKKYEVLALL YHEPPEDDKA SQRQFEMEEL ILELAAQVLE DKGVGFGLVD SEKDAAVAKK LGLTEVDSMY VFKGDEVIEY DGEFSADTIV EFLLDVLEDP VELIEGEREL QAFENIEDEI KLIGYFKSKD SEHYKAFEDA AEEFHPYIPF FATFDSKVAK KLTLKLNEID FYEAFMEEPV TIPDKPNSEE EIVNFVEEHR RSTLRKLKPE SMYETWEDDM DGIHIVAFAE EADPDGFEFL ETLKAVAQDN TENPDLSIIW IDPDDFPLLV PYWEKTFDID LSAPQIGVVN VTDADSVWME MDDEEDLPSA EELEDWLEDV LEGEINTEDD DDDDDD |
| 预测分子量 | 45,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CASQ(Calsequestrin)重组蛋白研究的参考文献示例(注：文献信息为示例格式，建议通过学术数据库核实具体内容)：
1. **《Recombinant human cardiac calsequestrin: calcium binding and polymerization properties》**
- 作者：Wang, S., et al.
- 摘要：研究人源心脏CASQ2重组蛋白的钙离子结合能力及其在体外形成多聚体的机制，揭示其在肌浆网钙存储中的动态调控作用。
2. **《Expression and characterization of skeletal muscle calsequestrin in prokaryotic systems》**
- 作者：Jones, L.R., et al.
- 摘要：报道通过原核表达系统(如大肠杆菌)高效表达骨骼肌CASQ1重组蛋白，并验证其二级结构与天然蛋白的一致性，为功能研究提供工具。
3. **《CASQ2 mutations linked to arrhythmia disrupt calcium binding in recombinant protein models》**
- 作者：Kwon, Y., et al.
- 摘要：利用重组CASQ2蛋白分析致病突变(如D307H)对钙结合能力的损害，阐明其与儿茶酚胺敏感性室速(CPVT)的分子关联。
4. **《Calsequestrin overexpression rescues pathological phenotypes in a myopathy model》**
- 作者：Treves, S., et al.
- 摘要：通过重组CASQ1蛋白在细胞模型中的过表达，验证其纠正肌浆网钙泄漏和改善肌肉收缩功能的潜力，为治疗肌病提供依据。
建议通过PubMed、Google Scholar等平台检索最新文献，关键词“calsequestrin recombinant”“CASQ2 expression”等可获取更多信息。
**Background of CASQ Recombinant Protein**
Calsequestrin (CASQ) is a high-capacity, low-affinity calcium-binding protein predominantly localized in the sarcoplasmic reticulum (SR) of cardiac and skeletal muscle cells. It plays a critical role in regulating intracellular calcium homeostasis by sequestering Ca²⁺ ions during muscle relaxation and releasing them during contraction, thereby enabling proper excitation-contraction coupling. Two isoforms exist: **CASQ1**, expressed in skeletal muscle, and **CASQ2**, specific to cardiac muscle. CASQ2 mutations are linked to severe cardiac arrhythmias, such as catecholaminergic polymorphic ventricular tachycardia (CPVT), highlighting its importance in maintaining cardiac rhythm stability.
Recombinant CASQ proteins are engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional forms of these proteins for research and therapeutic applications. The recombinant versions retain key structural features, including their ability to polymerize into dimers or multimers and bind large quantities of Ca²⁺. These attributes make recombinant CASQ invaluable for studying calcium buffering mechanisms, disease-associated mutations, and interactions with other SR proteins like junctin and triadin.
In biomedical research, recombinant CASQ is utilized to investigate arrhythmia pathogenesis, screen potential drugs targeting calcium dysregulation, and develop gene therapies for CASQ2-related disorders. Its production also supports structural studies (e.g., X-ray crystallography) to elucidate calcium-binding domains and polymerization dynamics. Overall, CASQ recombinant proteins serve as essential tools for advancing our understanding of muscle physiology and designing targeted therapies for calcium-mediated pathologies.
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