| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTX2 |
| Uniprot No | O75431 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-263aa |
| 氨基酸序列 | MSLVAEAFVSQIAAAEPWPENATLYQQLKGEQILLSDNAASLAVQAFLQMCNLPIKVVCRANAEYMSPSGKVPFIHVGNQVVSELGPIVQFVKAKGHSLSDGLEEVQKAEMKAYMELVNNMLLTAELYLQWCDEATVGEITHARYGSPYPWPLNHILAYQKQWEVKRKMKAIGWGKKTLDQVLEDVDQCCQALSQRLGTQPYFFNKQPTELDALVFGHLYTILTTQLTNDELSEKVKNYSNLLAFCRRIEQHYFEDRGKGRLS |
| 预测分子量 | 56.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于MTX2(Metaxin 2)重组蛋白研究的假设性参考文献示例(仅供参考，实际文献需通过学术数据库检索)：
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1. **文献名称**: *"Recombinant MTX2 Protein Expression and Its Role in Mitochondrial Membrane Dynamics"*
**作者**: Armstrong et al. (2018)
**摘要**: 研究利用大肠杆菌系统表达重组MTX2蛋白，并验证其在线粒体外膜蛋白转运中的作用，发现MTX2与TOM复合体互作，调控前体蛋白的输入效率。
2. **文献名称**: *"Structural Characterization of Human Metaxin-2 Using Recombinant Protein Technology"*
**作者**: Zhang et al. (2020)
**摘要**: 通过昆虫细胞系统纯化重组MTX2蛋白，结合X射线晶体学解析其三维结构，揭示了MTX2的C端结构域在结合线粒体伴侣蛋白中的关键作用。
3. **文献名称**: *"MTX2 Recombinant Protein as a Novel Biomarker for Apoptosis Detection"*
**作者**: Klein et al. (2019)
**摘要**: 开发基于重组MTX2蛋白的体外检测方法，证明其在细胞凋亡过程中释放至胞质，并与caspase-9存在直接相互作用，提示其作为凋亡标志物的潜力。
4. **文献名称**: *"Optimization of MTX2 Recombinant Protein Production and Stability Analysis"*
**作者**: Sato et al. (2021)
**摘要**: 对比不同表达系统(原核/真核)制备重组MTX2蛋白的产量及活性，发现哺乳动物细胞表达的蛋白具有更优的溶解性和功能稳定性，适用于药物筛选研究。
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**注意**：以上为假设文献，实际研究中请通过PubMed、Web of Science或Google Scholar检索关键词(如“MTX2 recombinant protein”“Metaxin-2 expression”)获取真实文献。
MTX2 recombinant protein is a engineered form of the Metaxin-2 (MTX2) protein, produced through molecular cloning and expression in heterologous systems like *E. coli* or mammalian cell cultures. MTX2. a less-studied member of the metaxin protein family, is localized to mitochondria-associated membranes (MAMs) and plays roles in mitochondrial function, including membrane dynamics, apoptosis regulation, and potential involvement in cholesterol transport. Its interaction with metaxin-1 and other mitochondrial proteins suggests a contribution to maintaining mitochondrial integrity and cellular energy metabolism.
Recombinant MTX2 is typically generated by inserting the MTX2 gene into expression vectors, followed by purification using affinity chromatography (e.g., His-tag systems). This allows researchers to obtain high-purity protein for functional studies. Current research focuses on its potential links to neurodegenerative diseases and cancer, as mitochondrial dysfunction is implicated in these conditions. For instance, altered MTX2 expression has been observed in certain tumors, though its exact mechanism remains unclear.
The protein’s applications include *in vitro* binding assays to map interaction partners, structural studies to elucidate its role in mitochondrial membrane architecture, and as an antigen for antibody development. Challenges in production include maintaining proper post-translational modifications when using prokaryotic systems, prompting some studies to employ eukaryotic expression platforms. As mitochondrial biology gains therapeutic attention, MTX2 recombinant protein serves as a tool to explore organelle-specific pathways and identify novel drug targets.
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