| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IPO11 |
| Uniprot No | Q9UI26 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-975aa |
| 氨基酸序列 | MDLNSASTVVLQVLTQATSQDTAVLKPAEEQLKQWETQPGFYSVLLNIFTNHTLDINVRWLAVLYFKHGIDRYWRRVAPHALSEEEKTTLRAGLITNFNEPINQIATQIAVLIAKVARLDCPRQWPELIPTLIESVKVQDDLRQHRALLTFYHVTKTLASKRLAADRKLFYDLASGIYNFACSLWNHHTDTFLQEVSSGNEAAILSSLERTLLSLKVLRKLTVNGFVEPHKNMEVMGFLHGIFERLKQFLECSRSIGTDNVCRDRLEKTIILFTKVLLDFLDQHPFSFTPLIQRSLEFSVSYVFTEVGEGVTFERFIVQCMNLIKMIVKNYAYKPSKNFEDSSPETLEAHKIKMAFFTYPTLTEICRRLVSHYFLLTEEELTMWEEDPEGFTVEETGGDSWKYSLRPCTEVLFIDIFHEYNQTLTPVLLEMMQTLQGPTNVEDMNALLIKDAVYNAVGLAAYELFDSVDFDQWFKNQLLPELQVIHNRYKPLRRRVIWLIGQWISVKFKSDLRPMLYEAICNLLQDQDLVVRIETATTLKLTVDDFEFRTDQFLPYLETMFTLLFQLLQQVTECDTKMHVLHVLSCVIERVNMQIRPYVGCLVQYLPLLWKQSEEHNMLRCAILTTLIHLVQGLGADSKNLYPFLLPVIQLSTDVSQPPHVYLLEDGLELWLVTLENSPCITPELLRIFQNMSPLLELSSENLRTCFKIINGYIFLSSTEFLQTYAVGLCQSFCELLKEITTEGQVQVLKVVENALKVNPILGPQMFQPILPYVFKGIIEGERYPVVMSTYLGVMGRVLLQNTSFFSSLLNEMAHKFNQEMDQLLGNMIEMWVDRMDNITQPERRKLSALALLSLLPSDNSVIQDKFCGIINISVEGLHDVMTEDPETGTYKDCMLMSHLEEPKVTEDEEPPTEQDKRKKMLALKDPVHTVSLQQFIYEKLKAQQEMLGEQGFQSLMETVDTEIVTQLQEFLQGF |
| 预测分子量 | 112,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IPO11重组蛋白的示例参考文献(供参考，建议通过学术数据库核实最新研究)：
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1. **文献名称**: *"Expression and Functional Analysis of Recombinant IPO11 in Nuclear Transport"*
**作者**: Zhang Y, et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化了重组IPO11蛋白，验证其与核转运相关蛋白(如β-importin)的相互作用，证实IPO11在调控TGF-β信号通路中的核质转运功能。
2. **文献名称**: *"Structural Insights into IPO11-Mediated Protein Transport by Cryo-EM"*
**作者**: Lee S, et al.
**摘要**: 通过冷冻电镜解析了重组IPO11与RanGTP复合物的三维结构，揭示了其识别特定核定位信号(NLS)的分子机制，为靶向核转运的疾病治疗提供结构基础。
3. **文献名称**: *"IPO11 Dysregulation in Cancer: Role of Recombinant Protein in Cell Proliferation Assays"*
**作者**: Gupta R, et al.
**摘要**: 使用哺乳动物细胞表达的重组IPO11蛋白，证明其在肺癌细胞中异常高表达可促进细胞增殖，并通过抑制实验表明IPO11可能成为潜在治疗靶点。
4. **文献名称**: *"Recombinant IPO11 Interaction with SMAD Proteins: Implications for Cellular Signaling"*
**作者**: Müller P, et al.
**摘要**: 通过体外结合实验发现重组IPO11与SMAD4蛋白直接结合，调控其核转运效率，从而影响TGF-β信号通路的活性，提出IPO11在发育和肿瘤转移中的作用。
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**备注**：以上文献为示例性质，实际研究请通过PubMed、Google Scholar等平台以“IPO11”、“Importin-11”、“recombinant protein”等关键词检索最新成果。
**Background of IPO11 Recombinant Protein**
IPO11 (Importin-11) is a member of the importin β family of nuclear transport receptors, which play critical roles in shuttling proteins between the cytoplasm and nucleus. It specifically mediates the nuclear import of cargo proteins by recognizing nuclear localization signals (NLS). IPO11 is unique in its ability to transport ubiquitinated substrates, linking it to proteasomal degradation pathways and cellular quality control mechanisms.
Structurally, IPO11 contains characteristic HEAT repeats that facilitate interactions with cargo proteins and components of the nuclear pore complex. Studies highlight its role in regulating key cellular processes, including cell cycle progression, DNA repair, and signal transduction. For instance, IPO11 interacts with the CRL4A ubiquitin ligase complex, influencing Wnt/β-catenin signaling—a pathway often dysregulated in cancers.
Recombinant IPO11 protein is produced using expression systems like *E. coli* or mammalian cells, enabling researchers to study its biochemical properties, binding partners, and mechanisms in vitro. Its recombinant form is essential for structural studies (e.g., crystallography) and functional assays to dissect nuclear transport dynamics.
Dysregulation of IPO11 is implicated in diseases such as prostate cancer, where its overexpression correlates with tumor progression, and neurodegenerative disorders linked to defective protein trafficking. Recombinant IPO11 serves as a tool for drug discovery, aiming to modulate nuclear transport pathways or disrupt pathogenic interactions.
In summary, IPO11 recombinant protein provides a versatile platform to explore nuclear transport biology, disease mechanisms, and therapeutic targeting, reflecting its broad significance in cellular homeostasis and pathology.
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