| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSD17b2 |
| Uniprot No | P37059 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-387aa |
| 氨基酸序列 | MSTFFSDTAWICLAVPTVLCGTVFCKYKKSSGQLWSWMVCLAGLCAVCLL ILSPFWGLILFSVSCFLMYTYLSGQELLPVDQKAVLVTGGDCGLGHALCK YLDELGFTVFAGVLNENGPGAEELRRTCSPRLSVLQMDITKPVQIKDAYS KVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVT KTFLPLLRKSKGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLEL SKWGIKVASIQPGGFLTNIAGTSDKWEKLEKDILDHLPAEVQEDYGQDYI LAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICL AHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT |
| 预测分子量 | 42,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSD17B2重组蛋白的3篇代表性文献的简要总结(注：文献信息为模拟示例，具体研究请以实际数据库检索为准)：
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1. **文献名称**：*"Cloning and Expression of Human 17β-Hydroxysteroid Dehydrogenase Type 2 in E. coli: Enzymatic Characterization and Substrate Specificity"*
**作者**：Adamski J. et al.
**摘要**：该研究成功克隆了人源HSD17B2基因，并在大肠杆菌中表达重组蛋白。通过纯化获得高活性酶，证实其催化雌二醇向雌酮的氧化反应，并揭示了其对不同类固醇底物的选择性。
2. **文献名称**：*"Functional Analysis of Recombinant HSD17B2 in Steroid Hormone Metabolism: Implications for Hormone-Dependent Cancers"*
**作者**：Mäkinen M. et al.
**摘要**：研究利用哺乳动物细胞表达系统制备重组HSD17B2蛋白，发现其通过降低活性雄激素/雌激素水平抑制激素依赖性癌细胞增殖，提示其潜在治疗应用价值。
3. **文献名称**：*"Structural Insights into HSD17B2 Catalysis: X-ray Crystallography of the Recombinant Enzyme Complexed with NAD+"*
**作者**：Persson B. et al.
**摘要**：通过解析重组HSD17B2与辅酶NAD+的复合物晶体结构，揭示了其催化机制的关键结构域，为设计选择性抑制剂提供了分子基础。
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如需具体文献，建议在 **PubMed** 或 **Web of Science** 中使用关键词 **"HSD17B2 recombinant protein"** 或 **"17β-HSD2 expression"** 检索最新研究。
HSD17B2 (17β-hydroxysteroid dehydrogenase type 2) is a member of the hydroxysteroid dehydrogenase family involved in steroid metabolism. This enzyme catalyzes the oxidation of active steroid hormones into their less active or inactive forms, playing a critical role in regulating hormone bioavailability. Specifically, HSD17B2 converts potent estradiol (E2) to estrone (E1) and testosterone to androstenedione, while also inactivating cortisol to cortisone. Its activity influences endocrine balance in tissues such as the placenta, liver, prostate, and endometrium, impacting processes like pregnancy maintenance, hormone-dependent cancers, and metabolic disorders.
Recombinant HSD17B2 protein is produced through genetic engineering for research and therapeutic applications. By expressing the HSD17B2 gene in heterologous systems (e.g., E. coli, insect cells), scientists obtain purified enzyme to study its structure-function relationships, substrate specificity, and regulatory mechanisms. The protein typically retains its NAD+-dependent oxidoreductase activity and membrane-binding properties observed in vivo. Researchers use it to investigate HSD17B2's role in hormone-sensitive pathologies, including breast cancer, endometriosis, and polycystic ovary syndrome (PCOS), where dysregulated hormone metabolism is implicated.
Current studies focus on developing HSD17B2 modulators as potential therapeutics. Recombinant protein assays enable high-throughput screening of inhibitors or activators that could fine-tune local hormone levels without systemic side effects. Structural analyses via crystallography or cryo-EM using recombinant HSD17B2 have revealed substrate-binding domains and catalytic residues, informing drug design. Challenges remain in maintaining enzyme stability during purification and replicating its native membrane-associated conformation. Nevertheless, recombinant HSD17B2 remains essential for decoding its physiological roles and exploring clinical interventions targeting steroid metabolism pathways.
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