| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | gABRb1 |
| Uniprot No | P18505 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-136aa |
| 氨基酸序列 | TNEPSNMPYVKETVDRLLKGYDIRLRPDFGGPPVDVGMRIDVASIDMVSE VNMDYTLTMYFQQSWKDKRLSYSGIPLNLTLDNRVADQLWVPDTYFLNDK KSFVHGVTV |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GABRB1重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **《Expression and Purification of Recombinant GABRB1 Protein in Mammalian Cells》**
- 作者：Smith A, et al.
- 摘要：研究通过哺乳动物表达系统(HEK293细胞)成功表达并纯化了GABRB1重组蛋白，证实其与GABA_A受体其他亚基的组装能力，为体外受体功能研究提供工具。
2. **《Structural Insights into GABRB1 Subunit Function via Recombinant Protein Crystallography》**
- 作者：Lee C, Zhang Y.
- 摘要：利用重组GABRB1蛋白进行X射线晶体学分析，揭示了其跨膜结构域的关键氨基酸位点，阐明了其在GABA结合及氯离子通道调控中的作用机制。
3. **《Role of Recombinant GABRB1 in Epilepsy-associated Mutations》**
- 作者：Wang H, et al.
- 摘要：通过体外表达携带癫痫相关突变的GABRB1重组蛋白，发现突变导致受体脱敏速率加快，提示GABRB1异常可能与神经兴奋性失调相关。
4. **《Development of a GABRB1-specific Nanobody using Recombinant Antigen》**
- 作者：Garcia R, et al.
- 摘要：以重组GABRB1蛋白为抗原筛选出高亲和力纳米抗体，可用于活细胞中GABA_A受体的动态成像及靶向治疗研究。
注：以上为模拟文献，实际研究需通过PubMed、Web of Science等平台检索关键词(如“GABRB1 recombinant protein”、“GABA receptor beta1 subunit”)获取。
**Background of GABRB1 Recombinant Protein**
The gamma-aminobutyric acid type A receptor beta 1 subunit (GABRB1) is a critical component of GABAA receptors, ligand-gated chloride channels that mediate fast inhibitory neurotransmission in the central nervous system. These receptors are pentameric structures typically composed of combinations of α, β, γ, δ, or ε subunits. The β1 subunit, encoded by the *GABRB1* gene, plays a pivotal role in receptor assembly, ligand binding, and ion channel function.
Recombinant GABRB1 protein is engineered using molecular cloning techniques, often expressed in heterologous systems like mammalian cells (e.g., HEK293) or insect cells to ensure proper post-translational modifications and functional folding. This protein is essential for studying GABAA receptor biophysics, pharmacology, and pathophysiology. Researchers utilize it to investigate receptor subunit interactions, screen drugs targeting neuropsychiatric disorders (e.g., anxiety, epilepsy), and explore mutations linked to genetic epilepsies or neurodevelopmental conditions.
The production of recombinant GABRB1 enables detailed structural analyses (e.g., cryo-EM, X-ray crystallography) and functional assays (e.g., electrophysiology, radioligand binding), providing insights into receptor activation, allosteric modulation, and subtype-specific drug design. Its role in GABAergic signaling also makes it a biomarker candidate for neurological diseases. Continued research on GABRB1 contributes to advancing therapies for disorders associated with inhibitory neurotransmission deficits.
×
