| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCLK3 |
| Uniprot No | Q9C098 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-648aa |
| 氨基酸序列 | MGKEPLTLKS IQVAVEELYP NKARALTLAQ HSRAPSPRLR SRLFSKALKG DHRCGETETP KSCSEVAGCK AAMRHQGKIP EELSLDDRAR TQKKWGRGKW EPEPSSKPPR EATLEERHAR GEKHLGVEIE KTSGEIIRCE KCKRERELQQ SLERERLSLG TSELDMGKGP MYDVEKLVRT RSCRRSPEAN PASGEEGWKG DSHRSSPRNP TQELRRPSKS MDKKEDRGPE DQESHAQGAA KAKKDLVEVL PVTEEGLREV KKDTRPMSRS KHGGWLLREH QAGFEKLRRT RGEEKEAEKE KKPCMSGGRR MTLRDDQPAK LEKEPKTRPE ENKPERPSGR KPRPMGIIAA NVEKHYETGR VIGDGNFAVV KECRHRETRQ AYAMKIIDKS RLKGKEDMVD SEILIIQSLS HPNIVKLHEV YETDMEIYLI LEYVQGGDLF DAIIESVKFP EPDAALMIMD LCKALVHMHD KSIVHRDLKP ENLLVQRNED KSTTLKLADF GLAKHVVRPI FTVCGTPTYV APEILSEKGY GLEVDMWAAG VILYILLCGF PPFRSPERDQ DELFNIIQLG HFEFLPPYWD NISDAAKDLV SRLLVVDPKK RYTAHQVLQH PWIETAGKTN TVKRQKQVSP SSEGHFRSQH KRVVEQVS |
| 预测分子量 | 73,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCLK3重组蛋白的模拟参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*"Recombinant DCLK3 Protein Modulates Neuronal Differentiation in vitro"*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌表达系统制备重组DCLK3蛋白，发现其通过调控微管稳定性促进神经干细胞分化，暗示DCLK3在神经发育中的潜在作用。
2. **文献名称**：*"Structural Characterization of DCLK3 Kinase Domain and Its Implications for Cancer Therapy"*
**作者**：Chen L, et al.
**摘要**：利用X射线晶体学解析DCLK3重组蛋白激酶结构域的三维结构，揭示其ATP结合口袋的独特特征，为设计靶向抑制剂提供依据。
3. **文献名称**：*"DCLK3 Recombinant Protein as a Biomarker in Colorectal Cancer Progression"*
**作者**：Wang Y, et al.
**摘要**：通过真核表达系统获得高纯度DCLK3重组蛋白，验证其在结直肠癌患者血清中的过表达，并证明其与肿瘤转移呈正相关。
4. **文献名称**：*"Functional Analysis of DCLK3 Isoforms Using Recombinant Proteins in Epithelial-Mesenchymal Transition"*
**作者**：Garcia R, et al.
**摘要**：比较不同剪接变体重组DCLK3蛋白的功能差异，发现长亚型通过激活Wnt/β-catenin通路显著增强上皮细胞迁移能力。
**注**：以上文献为示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。
**Background of DCLK3 Recombinant Protein**
DCLK3 (Doublecortin-like kinase 3) is a member of the doublecortin (DCX) domain-containing protein family, characterized by tandem doublecortin motifs at the N-terminus and a serine/threonine kinase domain at the C-terminus. It plays roles in microtubule binding, cytoskeletal regulation, and cellular processes such as neuronal development, cell migration, and mitosis. Dysregulation of DCLK3 has been implicated in neurological disorders, cancer progression, and ciliopathies, with studies suggesting its involvement in tumor invasiveness and stemness.
Recombinant DCLK3 protein is engineered using molecular cloning techniques, often expressed in systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. This purified protein serves as a critical tool for *in vitro* studies, enabling researchers to investigate its kinase activity, protein-protein interactions, and structural features. Applications include enzyme kinetics assays, screening for inhibitors or activators, and elucidating signaling pathways in disease models.
Recent research highlights DCLK3's potential as a therapeutic target, particularly in cancers where its overexpression correlates with poor prognosis. Its recombinant form also aids in antibody development for diagnostic purposes. Despite progress, challenges remain in understanding isoform-specific functions and context-dependent roles across tissues. Ongoing studies aim to clarify its mechanistic contributions to diseases and exploit its recombinant protein for targeted therapies.
Overall, DCLK3 recombinant protein is a vital resource for bridging molecular insights with translational applications in biomedicine.
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