| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADAT2 |
| Uniprot No | Q7Z6V5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-191aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MEAKAAPKPA ASGACSVSAE ETEKWMEEAM HMAKEALENT EVPVGCLMVY NNEVVGKGRN EVNQTKNATR HAEMVAIDQV LDWCRQSGKS PSEVFEHTVL YVTVEPCIMC AAALRLMKIP LVVYGCQNER FGGCGSVLNI ASADLPNTGR PFQCIPGYRA EEAVEMLKTF YKQENPNAPK SKVRKKECQK S |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADAT2重组蛋白的3篇代表性文献摘要概括：
1. **"Structural basis of tRNA modification by the ADAT2/ADAT3 complex"**
- **作者**: Torres AG, et al.
- **摘要**: 研究解析了ADAT2与ADAT3形成的复合物结构，利用重组蛋白技术在大肠杆菌中表达并纯化复合物，揭示其催化tRNA特定位点腺苷转化为肌苷的分子机制。
2. **"Recombinant expression and functional characterization of human ADAT2 in eukaryotic systems"**
- **作者**: Ramos JS, et al.
- **摘要**: 报道了人源ADAT2在昆虫细胞中的重组表达与纯化方法，证实重组蛋白具有酶活，并发现其依赖ADAT3结合才能稳定发挥tRNA编辑功能。
3. **"ADAT2 mutations impair tRNA wobble modification and cause intellectual disability"**
- **作者**: Alazami AM, et al.
- **摘要**: 通过重组突变体ADAT2蛋白实验，证明某些遗传突变导致其与ADAT3互作缺陷，影响tRNA修饰，进而引发神经发育异常，为疾病机制提供分子依据。
(注：上述文献标题及作者为虚拟示例，实际研究需通过学术数据库检索。)
ADAT2 (Adenosine Deaminase Acting on tRNA 2) is a eukaryotic enzyme critical for post-transcriptional modification of transfer RNA (tRNA). It forms a heterodimeric complex with ADAT3. catalyzing the deamination of adenosine to inosine at the wobble position (position 34) of specific tRNA anticodons. This editing process, known as A-to-I RNA editing, expands the decoding capacity of tRNAs, allowing a single tRNA to recognize multiple codons during translation. This mechanism enhances translational efficiency and accuracy, particularly for codons sharing the first two nucleotides. ADAT2-dependent modifications are essential for proper protein synthesis and are conserved across eukaryotes, highlighting their fundamental role in gene expression regulation.
Recombinant ADAT2 protein is engineered for biochemical and structural studies to elucidate its enzymatic mechanism, substrate specificity, and interaction with ADAT3. Produced using expression systems like *E. coli* or insect cells, the recombinant protein retains catalytic activity and enables *in vitro* analysis of its deaminase function. Research has linked ADAT2 dysfunction to human diseases, including neurodevelopmental disorders. Mutations in ADAT2/ADAT3 genes are associated with intellectual disability and microcephaly, likely due to disrupted tRNA modification and subsequent translational errors in critical neuronal proteins. Recombinant ADAT2 facilitates drug screening to identify potential therapeutics targeting these disorders. Additionally, it serves as a tool to study evolutionary aspects of RNA editing and its role in cellular stress responses. The protein’s solubility, stability, and purity (often verified via affinity tags like His-tag) make it valuable for X-ray crystallography or cryo-EM to resolve its 3D structure, advancing understanding of tRNA-modifying enzymes. Its applications extend to synthetic biology, where engineered tRNAs with optimized wobble modifications could improve recombinant protein production.
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