| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHgB1 |
| Uniprot No | Q9Y5G3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-927aa |
| 氨基酸序列 | MQRAREAEMMKSQVLFPFLLSLFCGAISQQIRYTIPEELANGSRVGKLAKDLGLSVRELPTRKLRVSAEDYFNVSLESGDLLVNGRIDREKICGRKLECALEFETVAENPMNVFHVVVVIQDINDNAPRFVAKGIDLEICESALPGVKFSLDSAQDADVEGNSLKLYTINPNQYFSLSTKESPDGSKYPVLLLEKPLDREHQSSHRLILTAMDGGDPPLSGTTHIWIRVTDANDNAPVFSQEVYRVSLQENVPWGTSVLRVMATDQDEGINAEITYAFLNSPISTSLFNLNPNTGDITTNGTLDFEETSRYVLSVEAKDGGVHTAHCNVQIEIVDENDNAPEVTFMSFSNQIPEDSDLGTVIALIKVRDKDSGQNGMVTCYTQEEVPFKLESTSKNYYKLVIAGALNREQTADYNVTIIATDKGKPALSSRTSITLHISDINDNAPVFHQASYVVHVSENNPPGASIAQVSASDPDLGPNGRVSYSILASDLEPRELLSYVSVSPQSGVVFAQRAFDHEQLRAFELTLQARDQGSPALSANVSLRVLVGDLNDNAPRVLYPALGPDGSALFDMVPRAAEPGYLVTKVVAVDADSGHNAWLSYHVLQASEPGLFSLGLRTGEVRTARALGDRDAARQRLLVAVRDGGQPPLSATATLHLIFADSLQEVLPDLSDRPEPSDPQTELQFYLVVALALISVLFLLAVILAIALRLRRSSSLDTEGCFQTGLCSKSGPGVPPNHSEGTLPYSYNLCIASHSAKTEFNSLNLTPEMAPPQDLLCDDPSMVVCASNEDHKIAYDPSLSSHQAPPNTDWRFSQAQRPGTSGSQNGDDTGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK |
| 预测分子量 | 100,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHgB1(原钙黏蛋白γ亚家族B1)重组蛋白的模拟参考文献示例(实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "Recombinant PCDHgB1 protein regulates synaptic adhesion in neuronal development"
**作者**: Chen L, et al.
**摘要**: 研究通过构建PCDHgB1重组蛋白，验证其在体外神经元突触形成中的关键作用。实验表明，该蛋白通过与膜表面受体相互作用，调控突触粘附及轴突导向。
2. **文献名称**: "Expression and functional analysis of PCDHgB1 extracellular domain in cancer cell migration"
**作者**: Kim S, Park JH
**摘要**: 利用大肠杆菌系统表达PCDHgB1胞外域重组蛋白，发现其抑制肿瘤细胞迁移能力，提示其可能通过干扰细胞间信号通路影响转移。
3. **文献名称**: "Structural characterization of PCDHgB1 recombinant protein by cryo-EM"
**作者**: Zhang Y, et al.
**摘要**: 采用冷冻电镜解析PCDHgB1重组蛋白的三维结构，揭示其钙离子结合域构象变化，为理解原钙黏蛋白家族分子机制提供结构基础。
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**注意**：以上为模拟文献，实际研究中请通过**PubMed**或**Google Scholar**以关键词“PCDHgB1 recombinant protein”检索最新成果。如需特定研究方向(如神经科学/癌症)，可进一步筛选文献。
**Background of PCDHGB1 Recombinant Protein**
Protocadherin gamma subfamily B1 (PCDHGB1) is a member of the clustered protocadherin-γ family, a group of transmembrane proteins critical for neural development, synaptic specificity, and neuronal connectivity. These proteins are characterized by extracellular cadherin (EC) repeats that mediate calcium-dependent cell-cell adhesion, a transmembrane domain, and a conserved cytoplasmic tail involved in intracellular signaling. The protocadherin-γ cluster, located on human chromosome 5. comprises 22 genes divided into A, B, and C subfamilies. PCDHGB1 belongs to the γB subfamily, which exhibits unique extracellular domains that enable diverse homophilic and heterophilic interactions, contributing to neuronal circuit assembly and synaptic plasticity.
Recombinant PCDHGB1 protein is engineered to study its structural and functional roles in vitro or in vivo. Produced via expression systems like *E. coli* or mammalian cells, the recombinant form retains key domains, including EC repeats and the cytoplasmic region, enabling investigations into adhesion mechanics, signaling pathways (e.g., interactions with tyrosine kinases or cytoskeletal regulators), and its influence on neurite outgrowth or dendritic arborization. Dysregulation of PCDHGB1 has been implicated in neurodevelopmental disorders, including autism spectrum disorders and schizophrenia, making it a potential biomarker or therapeutic target.
Research utilizing PCDHGB1 recombinant protein often focuses on deciphering its role in neural network formation, synaptic maintenance, and disease mechanisms. Its application spans protein interaction assays, antibody development, and functional studies in neuronal cultures or animal models. By elucidating PCDHGB1’s molecular interactions, researchers aim to uncover novel insights into brain development and pathology, bridging gaps between genetic variation and neurological dysfunction.
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