Recombinant Human ATP11B protein

**Background of ATP11B Recombinant Protein**

ATP11B is a member of the P4-type ATPase family, a group of transmembrane enzymes critical for maintaining phospholipid asymmetry in biological membranes. These proteins act as lipid flippases, utilizing energy from ATP hydrolysis to translocate specific phospholipids (e.g., phosphatidylserine) from the outer to the inner leaflet of cell membranes. This activity is essential for cellular processes such as vesicle trafficking, membrane repair, and apoptosis regulation.

The ATP11B gene, located on human chromosome 3. encodes a multi-pass membrane protein predominantly expressed in tissues like the brain, liver, and testes. Dysregulation of ATP11B has been linked to neurological disorders, cancer progression, and metabolic diseases, highlighting its physiological importance. Recombinant ATP11B protein is engineered using heterologous expression systems (e.g., mammalian or insect cell lines) to ensure proper post-translational modifications and functional fidelity.

Purified recombinant ATP11B is widely used in biochemical studies to dissect its flippase activity, substrate specificity, and interaction partners. Its structure-function relationship is explored via mutagenesis or cryo-EM to identify critical domains for lipid binding and ATP hydrolysis. Additionally, this protein serves as a tool in drug discovery, enabling screens for modulators targeting membrane-related pathologies. Research on ATP11B also contributes to understanding broader mechanisms of cellular membrane homeostasis and disease-associated lipid dysregulation.

In summary, ATP11B recombinant protein is a vital reagent for unraveling the molecular basis of lipid transport and its implications in health and disease.