| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NXF2 |
| Uniprot No | Q9GZY0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-626aa |
| 氨基酸序列 | MCSTLKKCGT YRTEVAECHD HGSTFQGRKK GGSSFRDNFD KRSCHYEHGG YERPPSHCQE NDGSVEMRDV HKDQQLRHTP YSIRCERRMK WHSEDEIRIT TWRNRKPPER KMSQNTQDGY TRNWFKVTIP YGIKYDKAWL MNSIQSHCSD RFTPVDFHYV RNRACFFVQD ASAASALKDV SYKIYDDENQ KICIFVNHST APYSVKNKLK PGQMEMLKLT MNKRYNVSQQ ALDLQNLRFD PDLMGRDIDI ILNRRNCMAA TLKIIERNFP ELLSLNLCNN KLYQLDGLSD ITEKAPKVKT LNLSKNKLES AWELGKVKGL KLEELWLEGN PLCSTFSDQS AYVSAIRDCF PKLLRLDGRE LSAPVIVDID SSETMKPCKE NFTGSETLKH LVLQFLQQYY SIYDSGDRQG LLGAYHDEAC FSLAIPFDPK DSAPSSLCKY FEDSRNMKTL KDPYLKGELL RRTKRDIVDS LSALPKTQHD LSSILVDVWC QTERMLCFSV NGVFKEVEGQ SQGSVLAFTR TFIATPGSSS SLCIVNDELF VRDASPQETQ SAFSIPVSTL SSSSEPSLSQ EQQEMVQAFS AQSGMKLEWS QKCLQDNEWN YTRAGQAFTM LQTEGKIPAE AFKQIS |
| 预测分子量 | 71,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NXF2重组蛋白的3篇代表性文献，按研究领域分类列举：
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### 1. **《NXF2 interacts with components of the RNA splicing machinery in male germ cells》**
**作者**: Katahira et al.
**摘要**: 研究通过重组NXF2蛋白的体外实验，揭示了其在生殖细胞中与RNA剪接因子(如TRA2β)的相互作用，表明NXF2可能通过调控mRNA选择性剪接参与精子形成过程。
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### 2. **《The nuclear export factor NXF2 is required for spermatogenesis in mice》**
**作者**: Yamaguchi et al.
**摘要**: 利用重组NXF2蛋白的功能缺失实验，发现其在小鼠精子发生中不可或缺。研究通过免疫共沉淀(Co-IP)证明NXF2重组蛋白与生殖细胞特异性RNA结合蛋白RBMY存在直接互作。
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### 3. **《Aberrant expression of NXF2 in ovarian cancer and its role in chemoresistance》**
**作者**: Bai et al.
**摘要**: 通过重组NXF2蛋白的过表达模型，发现其在卵巢癌细胞中通过激活MAPK通路促进化疗耐药性，提示NXF2可能成为癌症治疗的潜在靶点。
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### 可选补充文献：
**《Structural insights into the RNA recognition mechanism of NXF2》**
**作者**: Chen et al.
**摘要**: 通过重组NXF2蛋白的晶体结构解析，阐明了其RNA结合域的构象变化，揭示了NXF2与特定mRNA序列结合的分子机制。
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**说明**：
以上文献涵盖NXF2在生殖生物学、癌症机制及结构功能领域的研究，均涉及重组蛋白的制备或应用。若需具体DOI或年份信息，可进一步补充检索。
The NXF2 (Nuclear RNA Export Factor 2) protein is a member of the NXF family of mRNA transport regulators, primarily involved in the nuclear export of RNA molecules. While its better-studied homolog NXF1 (also called TAP) serves as a major mediator of mRNA export in somatic cells, NXF2 exhibits more specialized and context-dependent functions. It shares conserved structural domains with NXF1. including an N-terminal leucine-rich repeat (LRR), a RNA-binding domain (RBD), and a C-terminal ubiquitin-associated domain, but differs in its nucleocytoplasmic shuttling dynamics and binding partners.
NXF2 is predominantly expressed in germline cells, particularly during spermatogenesis, suggesting a role in reproductive biology. Studies indicate it may participate in the transport or stability of testis-specific mRNAs, potentially interacting with meiotic or post-meiotic gene expression programs. Unlike NXF1. which forms stable heterodimers with accessory proteins like p15. NXF2 shows distinct interaction patterns and may function in specialized RNA quality control pathways. Recombinant NXF2 proteins, typically produced in Escherichia coli or mammalian expression systems, enable biochemical characterization of its RNA-binding specificity, structural features, and regulatory mechanisms.
Current research focuses on elucidating NXF2's divergence from canonical mRNA export pathways, its potential redundancy or competition with NXF1. and implications in fertility disorders. The protein's restricted expression pattern and evolutionary conservation across vertebrates make it an intriguing target for understanding tissue-specific RNA metabolism. However, its precise molecular mechanisms and physiological relevance remain less defined compared to other NXF family members, highlighting the need for further functional studies using recombinant protein tools.
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