| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAS8 |
| Uniprot No | O95995 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-478aa |
| 氨基酸序列 | MAPKKKGKKG KAKGTPIVDG LAPEDMSKEQ VEEHVSRIRE ELDREREERN YFQLERDKIH TFWEITRRQL EEKKAELRNK DREMEEAEER HQVEIKVYKQ KVKHLLYEHQ NNLTEMKAEG TVVMKLAQKE HRIQESVLRK DMRALKVELK EQELASEVVV KNLRLKHTEE ITRMRNDFER QVREIEAKYD KKMKMLRDEL DLRRKTELHE VEERKNGQIH TLMQRHEEAF TDIKNYYNDI TLNNLALINS LKEQMEDMRK KEDHLEREMA EVSGQNKRLA DPLQKAREEM SEMQKQLANY ERDKQILLCT KARLKVREKE LKDLQWEHEV LEQRFTKVQQ ERDELYRKFT AAIQEVQQKT GFKNLVLERK LQALSAAVEK KEVQFNEVLA ASNLDPAALT LVSRKLEDVL ESKNSTIKDL QYELAQVCKA HNDLLRTYEA KLLAFGIPLD NVGFKPLETA VIGQTLGQGP AGLVGTPT |
| 预测分子量 | 56,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAS8重组蛋白的3篇参考文献的简要概括：
1. **"GAS8 and its splice variant mediate cellular functions through microtubule-based structures"**
- 作者：Y. Watanabe et al.
- 摘要：研究报道GAS8重组蛋白在体外促进微管结构的稳定性和纤毛形成，揭示其通过调控微管动力学参与细胞运动和信号传导。
2. **"Functional characterization of the GAS8-AS1 lncRNA and its interaction with the GAS8 protein in prostate cancer"**
- 作者：R. Chen et al.
- 摘要：通过重组GAS8蛋白实验，发现其与长链非编码RNA GAS8-AS1结合，抑制前列腺癌细胞增殖并诱导凋亡，提示其肿瘤抑制潜力。
3. **"Recombinant GAS8 protein restores ciliary motility in a zebrafish model of primary ciliary dyskinesia"**
- 作者：L. Smith et al.
- 摘要：研究利用重组GAS8蛋白在斑马鱼模型中恢复纤毛运动功能，验证GAS8作为动力蛋白复合物关键组分在纤毛相关疾病中的作用。
(注：以上文献为示例，实际引用需核对真实存在的论文。)
**Background of GAS8 Recombinant Protein**
GAS8 (Growth Arrest-Specific 8) is a member of the growth arrest-specific gene family, initially identified for its upregulation during cellular growth arrest. It encodes a protein involved in regulating cytoskeletal dynamics, ciliary motility, and intracellular signaling pathways. GAS8 is evolutionarily conserved and shares homology with components of the axonemal dynein complex, which drives the movement of cilia and flagella. Structurally, GAS8 contains a conserved N-terminal domain and a coiled-coil region, facilitating interactions with other proteins such as the DYX1C1 and DNAI2. critical for proper ciliary function.
Dysregulation of GAS8 has been implicated in primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance, recurrent respiratory infections, and infertility. Additionally, GAS8 is linked to cancer progression, where it may act as a tumor suppressor or promoter depending on the cellular context, influencing cell migration, apoptosis, and metastasis.
Recombinant GAS8 protein is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable functional studies. Its purified form allows researchers to investigate interactions with binding partners, enzymatic activities, and roles in ciliary assembly or signaling pathways like Hedgehog. Recombinant GAS8 also serves as an antigen for antibody development and a tool for high-throughput drug screening targeting ciliopathies or cancer.
Overall, GAS8 recombinant protein provides a vital resource for dissecting its biological roles and therapeutic potential in ciliary disorders and cancer.
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