| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP3b1 |
| Uniprot No | O00203 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 944-1094aa |
| 氨基酸序列 | SITVSMGIDFCDSTQTASFQLCTKDDCFNVNIQPPVGELLLPVAMSEKDFKKEQGVLTGMNETSAVIIAAPQNFTPSVIFQKVVNVANVGAVPSGQDNIHRFAAKTVHSGSLMLVTVELKEGSTAQLIINTEKTVIGSVLLRELKPVLSQG |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **AP3B1重组蛋白** 的3篇参考文献示例(内容基于研究领域常见方向，部分为虚构示例供参考)：
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1. **标题**: *"Expression and Functional Characterization of Recombinant AP3B1 in Adaptor Protein Complex Assembly"*
**作者**: Smith, J.R., Dell'Angelica, E.C., Bonifacino, J.S.
**摘要**: 研究通过大肠杆菌系统表达并纯化AP3B1重组蛋白，验证其与AP-3复合体其他亚基(如AP3D1)的相互作用，揭示其在溶酶体运输中的关键结构域。
2. **标题**: *"AP3B1 Mutations Disrupt Lysosomal Trafficking: Insights from Recombinant Protein Reconstitution"*
**作者**: Li, H., Raposo, G., Marks, M.S.
**摘要**: 构建AP3B1突变体重组蛋白，证明其在体外无法招募下游效应蛋白(如BLOC-1)，导致黑色素细胞中溶酶体相关细胞器生成缺陷，解释Hermansky-Pudlak综合征的分子机制。
3. **标题**: *"Structural Analysis of AP3B1 Reveals a Clathrin-Binding Motif Essential for Vesicle Formation"*
**作者**: Zhang, Y., Owen, D.J., Kirchhausen, T.
**摘要**: 利用X射线晶体学解析AP3B1重组蛋白的C端结构，鉴定出与网格蛋白(clathrin)结合的关键基序，为AP-3复合体介导的囊泡出芽机制提供结构证据。
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**备注**：以上文献为示例，实际研究中建议通过 **PubMed/Google Scholar** 检索关键词 *"AP3B1 recombinant protein"* 或 *"AP3B1 adaptor complex"* 获取真实文献。关注领域内权威期刊如 *Traffic*, *JBC*, *Cell Reports* 等。
**Background of AP3B1 Recombinant Protein**
AP3B1 (Adaptor-Related Protein Complex 3 Beta 1 Subunit) is a critical component of the heterotetrameric AP-3 complex, which regulates intracellular vesicle trafficking and cargo sorting to lysosome-related organelles (LROs). The AP-3 complex facilitates the transport of transmembrane proteins from early endosomes to LROs, such as melanosomes, platelet dense granules, and lytic granules in cytotoxic T-cells. AP3B1 encodes the β3A subunit of this complex, and mutations in this gene are linked to Hermansky-Pudlak syndrome type 2 (HPS2), a rare autosomal recessive disorder characterized by oculocutaneous albinism, immune dysfunction, and bleeding disorders due to LRO defects.
Recombinant AP3B1 protein is engineered to study its structural and functional roles in vesicular trafficking and disease mechanisms. Produced via expression systems like *E. coli* or mammalian cells, the recombinant protein retains binding domains essential for interacting with other AP-3 subunits (e.g., δ, μ3. σ3) and cargo recognition motifs. Its production often involves affinity tags (e.g., His-tag) for purification, followed by validation through Western blotting or functional assays.
Research utilizing AP3B1 recombinant protein focuses on elucidating molecular pathways disrupted in HPS2. exploring protein interactions within the AP-3 complex, and developing model systems to test therapeutic strategies, such as gene therapy or small-molecule correctors. Additionally, it aids in studying broader trafficking disorders and immune cell dysfunction, offering insights into lysosomal storage diseases and immune deficiencies. The protein’s application extends to drug discovery, serving as a target for restoring disrupted trafficking in HPS2 and related conditions.
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