| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP1m1 |
| Uniprot No | Q9BXS5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-423aa |
| 氨基酸序列 | SASAVYVLD LKGKVLICRN YRGDVDMSEV EHFMPILMEK EEEGMLSPIL AHGGVRFMWI KHNNLYLVAT SKKNACVSLV FSFLYKVVQV FSEYFKELEE ESIRDNFVII YELLDELMDF GYPQTTDSKI LQEYITQEGH KLETGAPRPP ATVTNAVSWR SEGIKYRKNE VFLDVIESVN LLVSANGNVL RSEIVGSIKM RVFLSGMPEL RLGLNDKVLF DNTGRGKSKS VELEDVKFHQ CVRLSRFEND RTISFIPPDG EFELMSYRLN THVKPLIWIE SVIEKHSHSR IEYMIKAKSQ FKRRSTANNV EIHIPVPNDA DSPKFKTTVG SVKWVPENSE IVWSIKSFPG GKEYLMRAHF GLPSVEAEDK EGKPPISVKF EIPYFTTSGI QVRYLKIIEK SGYQALPWVR YITQNGDYQL RTQ |
| 预测分子量 | 48,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP1m1重组蛋白的3条参考文献示例(内容基于学术文献框架归纳，非真实引用)：
1. **文献名称**：*AP1M1 regulates protein trafficking and cell polarity in epithelial cells*
**作者**：Smith J, et al.
**摘要**：研究通过表达重组AP1m1蛋白，揭示了其在调控上皮细胞蛋白质分选和细胞极性建立中的关键作用，证明AP1m1缺失会导致细胞间连接蛋白的错误定位。
2. **文献名称**：*Structural analysis of AP1M1 subunit reveals clathrin adaptor complex assembly mechanism*
**作者**：Lee H, et al.
**摘要**：利用重组AP1m1蛋白进行X射线晶体学分析，解析了其与AP1复合体其他亚基的相互作用界面，阐明了衔接蛋白复合体的组装机制。
3. **文献名称**：*AP1M1 mutations impair neuronal synaptic vesicle recycling and cause neurodevelopmental disorders*
**作者**：Wang Y, et al.
**摘要**：通过体外重组AP1m1蛋白功能挽救实验，证实其基因突变导致突触小泡循环障碍，与自闭症谱系障碍的病理机制相关。
注：以上为模拟文献，实际文献需通过PubMed/Google Scholar检索关键词"AP1M1 recombinant protein"或"AP1M1 function"获取。
**Background of AP1m1 Recombinant Protein**
AP1m1 (Adaptor Protein Complex 1 Subunit Mu-1) is a critical component of the heterotetrameric AP-1 complex, which belongs to the adaptor protein family involved in intracellular vesicle trafficking. The AP-1 complex facilitates the formation of clathrin-coated vesicles (CCVs) and mediates cargo sorting between the trans-Golgi network (TGN) and endosomal compartments. AP1m1. the μ1 subunit of AP-1. plays a pivotal role in recognizing sorting signals on cargo proteins, ensuring their proper packaging into transport vesicles.
Structurally, AP1m1 contains a conserved μ-homology domain that binds tyrosine-based sorting motifs (e.g., YXXΦ) on transmembrane cargos. Dysregulation of AP1m1 has been linked to impaired vesicular transport, affecting cellular processes such as protein secretion, receptor recycling, and lysosomal function. Mutations in the *AP1M1* gene are associated with rare genetic disorders, including MEDNIK syndrome (mental retardation, enteropathy, deafness, neuropathy, ichthyosis, and keratodermia) and other pathologies involving epithelial or neurological dysfunction.
Recombinant AP1m1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional forms for biochemical and cell biological studies. It serves as a tool to investigate AP-1 complex assembly, cargo interactions, and trafficking mechanisms. Researchers utilize AP1m1 recombinant proteins in pull-down assays, structural studies (e.g., X-ray crystallography), or functional reconstitution experiments to dissect its role in health and disease. Additionally, it aids in exploring therapeutic strategies targeting trafficking defects in cancers, neurodegenerative diseases, or genetic syndromes.
Overall, AP1m1 recombinant protein is vital for advancing our understanding of intracellular transport pathways and their implications in human disease.
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