| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXD3 |
| Uniprot No | Q9UJU5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-478aa |
| 氨基酸序列 | 29 amino acid Tag-GEFTLSGGGSASDMSGQTVLTAEDVDIDVVGEGDDGLEEKDSDAGC DSPAGPPEL RLDEADEVPPAAPHHGQPQPPHQQPLTLPKEAAGAGAGP GGDVGAPEADGCKGGVGGEEGGASG GGPGAGSGSAGGLAPSKPKNSLV KPPYSYIALITMAILQSPQKKLTLSGICEFISNRFPYYREK FPAWQNS IRHNLSLNDCFVKIPREPGNPGKGNYWTLDPQSEDMFDNGSFLRRRKRFK RHQQEHL REQTALMMQSFGAYSLAAAAGAAGPYGRPYGLHPAAAAGAY SHPAAAAAAAAAAALQYPYALPP VAPVLPPAVPLLPSGELGRKAAAFG SQLGPGLQLQLNSLGAAAAAAGTAGAAGTTASLIKSEPS ARPSFSIEN IIGGGPAAPGGSAVGAGVAGGTGGSGGGSTAQSFLRPPGTVQSAALMATH QPLSL SRTTATIAPILSVPLSGQFLQPAASAAAAAAAAAQAKWPAQLE ESGGGGSPGRRRRRRRRRRR |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXD3重组蛋白的3篇代表性参考文献的示例(注：文献为假设性概括，实际文献需通过数据库查询确认)：
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1. **"FOXD3 Recombinant Protein Suppresses Epithelial-Mesenchymal Transition in Cancer Cells"**
- **作者**: Zhang Y. et al.
- **摘要**: 研究通过表达并纯化FOXD3重组蛋白，证明其能够结合特定靶基因启动子，抑制TGF-β诱导的上皮间质转化(EMT)，降低癌细胞迁移能力，提示其在肿瘤治疗中的潜在应用。
2. **"Structural Insights into the DNA-Binding Domain of FOXD3"**
- **作者**: Lee S. & Kim J.H.
- **摘要**: 利用重组FOXD3蛋白进行X射线晶体学分析，解析其DNA结合域的三维结构，揭示关键氨基酸残基与靶DNA序列的相互作用机制，为突变导致的功能缺陷提供分子基础。
3. **"FOXD3 Recombinant Protein Enhances Pluripotency in Embryonic Stem Cells"**
- **作者**: Gupta R. et al.
- **摘要**: 研究发现重组FOXD3蛋白通过激活OCT4和NANOG等核心多能性基因，维持胚胎干细胞自我更新，且其敲除导致分化表型，表明其在干细胞调控中的核心作用。
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建议通过PubMed或Web of Science以“FOXD3 recombinant protein”为关键词检索最新文献，获取具体研究细节。
FOXD3. a member of the Forkhead box (FOX) family of transcription factors, is characterized by a conserved "forkhead" or "winged-helix" DNA-binding domain. This protein plays critical roles in embryonic development, stem cell pluripotency, and cellular differentiation. FOXD3 is notably expressed in early embryonic tissues, neural crest cells, and certain stem cell populations, where it regulates target genes by binding to specific DNA sequences. Studies highlight its dual functionality: it can either activate or repress transcription depending on cellular context, influencing processes like cell fate determination and tumor suppression. Dysregulation of FOXD3 has been linked to developmental disorders and cancer, with evidence suggesting both oncogenic and tumor-suppressive roles in different malignancies.
Recombinant FOXD3 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research applications. Its recombinant form often includes affinity tags (e.g., His-tag, GST) for simplified purification and detection. Researchers utilize this tool to investigate FOXD3's DNA-binding mechanics, protein-protein interactions, and regulatory pathways in vitro or in cell-based assays. In stem cell research, recombinant FOXD3 aids in exploring its role in maintaining pluripotency or driving differentiation. Cancer studies leverage it to dissect its context-dependent effects on tumor progression. The protein’s activity is typically validated through electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), or luciferase reporter systems. As a standardized reagent, recombinant FOXD3 enhances reproducibility in mechanistic studies and drug discovery efforts targeting FOX family-associated diseases.
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