| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP3d1 |
| Uniprot No | O14617 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1153aa |
| 氨基酸序列 | MALKMVKGSIDRMFDKNLQDLVRGIRNHKEDEAKYISQCIDEIKQELKQDNIAVKANAVCKLTYLQMLGYDISWAAFNIIEVMSASKFTFKRIGYLAASQSFHEGTDVIMLTTNQIRKDLSSPSQYDTGVALTGLSCFVTPDLARDLANDIMTLMSHTKPYIRKKAVLIMYKVFLKYPESLRPAFPRLKEKLEDPDPGVQSAAVNVICELARRNPKNYLSLAPLFFKLMTSSTNNWVLIKIIKLFGALTPLEPRLGKKLIEPLTNLIHSTSAMSLLYECVNTVIAVLISLSSGMPNHSASIQLCVQKLRILIEDSDQNLKYLGLLAMSKILKTHPKSVQSHKDLILQCLDDKDESIRLRALDLLYGMVSKKNLMEIVKKLMTHVDKAEGTTYRDELLTKIIDICSQSNYQYITNFEWYISILVELTRLEGTRHGHLIAAQMLDVAIRVKAIRKFAVSQMSALLDSAHLLASSTQRNGICEVLYAAAWICGEFSEHLQEPHHTLEAMLRPRVTTLPGHIQAVYVQNVVKLYASILQQKEQAGEAEGAQAVTQLMVDRLPQFVQSADLEVQERASCILQLVKHIQKLQAKDVPVAEEVSALFAGELNPVAPKAQKKVPVPEGLDLDAWINEPLSDSESEDERPRAVFHEEEQRRPKHRPSEADEEELARRREARKQEQANNPFYIKSSPSPQKRYQDTPGVEHIPVVQIDLSVPLKVPGLPMSDQYVKLEEERRHRQKLEKDKRRKKRKEKEKKGKRRHSSLPTESDEDIAPAQQVDIVTEEMPENALPSDEDDKDPNDPYRALDIDLDKPLADSEKLPIQKHRNTETSKSPEKDVPMVEKKSKKPKKKEKKHKEKERDKEKKKEKEKKKSPKPKKKKHRKEKEERTKGKKKSKKQPPGSEEAAGEPVQNGAPEEEQLPPESSYSLLAENSYVKMTCDIRGSLQEDSQVTVAIVLENRSSSILKGMELSVLDSLNARMARPQGSSVHDGVPVPFQLPPGVSNEAQYVFTIQSIVMAQKLKGTLSFIAKNDEGATHEKLDFRLHFSCSSYLITTPCYSDAFAKLLESGDLSMSSIKVDGIRMSFQNLLAKICFHHHFSVVERVDSCASMYSRSIQGHHVCLLVKKGENSVSVDGKCSDSTLLSNLLEEMKATLAKC |
| 预测分子量 | 130 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP3d1重组蛋白的模拟参考文献示例(建议通过PubMed或Google Scholar核实真实文献)：
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1. **标题**：*Structural and functional analysis of the AP-3 δ-subunit (AP3d1) in lysosomal trafficking*
**作者**：Benson MA, et al.
**摘要**：本研究通过重组表达人源AP3d1蛋白，解析其与AP-3复合体其他亚基的相互作用，揭示AP3d1在溶酶体靶向运输中的关键作用。实验表明，重组AP3d1可与β3亚基结合，并调控囊泡形成。
2. **标题**：*Recombinant AP3d1 restores cargo sorting in AP-3-deficient cells*
**作者**：Dell'Angelica EC, et al.
**摘要**：利用哺乳动物细胞系统表达重组AP3d1蛋白，证明其可挽救AP-3缺陷细胞的囊泡分选功能缺陷，特别是黑素体和血小板致密颗粒的生成，支持AP3d1在遗传性疾病(如Hermansky-Pudlak综合征)中的病理机制。
3. **标题**：*Expression and purification of the AP3d1 subunit for in vitro binding assays*
**作者**：Zhong L, et al.
**摘要**：报道了在大肠杆菌中高效表达并纯化重组AP3d1蛋白的方法，通过体外结合实验验证了其与细胞质内接头蛋白的相互作用，为AP-3复合体组装机制提供证据。
4. **标题**：*AP3d1 mutations disrupt synaptic vesicle protein sorting: Insights from recombinant protein rescue models*
**作者**：Park Y, et al.
**摘要**：通过构建AP3d1突变体重组蛋白，在神经元模型中证明特定突变导致突触小泡蛋白分选异常，强调了AP3d1在神经细胞内膜运输中的功能重要性。
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**注意**：以上文献为模拟示例，实际研究请检索权威数据库(如PubMed)。建议使用关键词“AP3d1 recombinant”“AP3 complex trafficking”进行精确查询。
**Background of AP3d1 Recombinant Protein**
AP3d1. a subunit of the Adaptor Protein complex 3 (AP-3), plays a critical role in intracellular vesicle trafficking and cargo sorting. The AP-3 complex, composed of β3. δ (AP3d1), μ3. and σ3 subunits, facilitates the formation of cargo-specific transport vesicles from the trans-Golgi network (TGN) and endosomes, directing proteins to lysosomes, lysosome-related organelles (LROs), and specialized compartments like melanosomes and platelet dense granules. AP3d1. encoded by the *AP3D1* gene, contributes to the complex’s structural integrity and mediates interactions with membrane lipids and cargo receptors.
Mutations in *AP3D1* are linked to Hermansky-Pudlak syndrome type 2 (HPS-2), a rare genetic disorder characterized by oculocutaneous albinism, immune dysfunction, and bleeding disorders due to defects in LRO biogenesis. This highlights AP3d1’s essential role in organelle maturation and cellular homeostasis.
Recombinant AP3d1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study AP-3’s molecular mechanisms. It retains functional domains, including the N-terminal trunk and β3-appendage region, enabling in vitro analyses of protein interactions, vesicle formation, and cargo recognition. Purified via affinity chromatography, the recombinant protein serves as a tool for structural studies (e.g., cryo-EM), biochemical assays, and disease modeling. Researchers utilize it to explore AP-3 dysfunction in neurodegenerative diseases (e.g., Alzheimer’s) and lysosomal storage disorders, as well as to screen therapeutic candidates targeting trafficking pathways. Its application advances understanding of cellular logistics and potential treatments for AP-3-related pathologies.
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