Recombinant Human TNKS1BP1 protein

TNKS1BP1 (Tankyrase 1 Binding Protein 1), also known as TAB182. is a multifunctional scaffold protein that interacts with tankyrase 1 (TNKS1), a member of the poly(ADP-ribose) polymerase (PARP) family. This interaction is mediated through its C-terminal sterile alpha motif (SAM) domain, which binds to the ankyrin repeat clusters of TNKS1. TNKS1BP1 plays a regulatory role in diverse cellular processes, including telomere maintenance, Wnt/β-catenin signaling, and genomic stability. It acts as a negative modulator of TNKS1 activity by competing with other substrates for binding, thereby influencing PARsylation-dependent pathways critical for cancer progression and stem cell regulation.

Recombinant TNKS1BP1 protein is engineered for in vitro studies to dissect its molecular interactions and therapeutic potential. Typically produced in bacterial or mammalian expression systems, the purified protein retains functional domains required for binding TNKS1 and other partners. Its applications span mechanistic studies of tankyrase-mediated processes, drug screening for PARP inhibitors, and structural analyses to map interaction interfaces. Researchers also utilize it to explore TNKS1BP1’s role in diseases; for instance, dysregulation of TNKS1-TNKS1BP1 axis is implicated in colorectal cancer (via Wnt pathway) and neurodegenerative disorders. However, challenges remain in resolving its full interactome and context-dependent functions. Current efforts focus on optimizing recombinant variants (e.g., truncated forms or tagged versions) to enhance solubility and experimental utility, supporting translational research targeting tankyrase-related pathologies.