| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAFB |
| Uniprot No | Q15424 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-915aa |
| 氨基酸序列 | MAETLSGLGDSGAAGAAALSSASSETGTRRLSDLRVIDLRAELRKRNVDSSGNKSVLMERLKKAIEDEGGNPDEIEITSEGNKKTSKRSSKGRKPEEEGVEDNGLEENSGDGQEDVETSLENLQDIDIMDISVLDEAEIDNGSVADCVEDDDADNLQESLSDSRELVEGEMKELPEQLQEHAIEDKETINNLDTSSSDFTILQEIEEPSLEPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASESTAHAQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSPEARDSKEDGRKFDFDACNEVPPAPKESSTSEGADQKMSSPEDDSDTKRLSKEEKGRSSCGRNFWVSGLSSTTRATDLKNLFSKYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCINHLHKTELHGKMISVEKAKNEPVGKKTSDKRDSDGKKEKSSNSDRSTNLKRDDKCDRKDDAKKGDDGSGEKSKDQDDQKPGPSERSRATKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKEPRKSRDSESHSRVRERSEREQRMQAQWEREERERLEIARERLAFQRQRLERERMERERLERERMHVEHERRREQERIHREREELRRQQELRYEQERRPAVRRPYDLDRRDDAYWPEAKRAALDERYHSDFNRQDRFHDFDHRDRGRYPDHSVDRREGSRSMMGEREGQHYPERHGGPERHGRDSRDGWGGYGSDKRMSEGRGLPPPPRRDWGDHGRREDDRSWQGTADGGMMDRDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHPIPHGGMQGGFGGQSRGSRPSDARFTRRY |
| 预测分子量 | 102,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与SAFB重组蛋白相关的3篇文献概览：
1. **"Scaffold Attachment Factor B (SAFB) is required for expression of pre-rRNA by RNA polymerase I"**
- **作者**: Oesterreich, S. et al.
- **摘要**: 研究揭示了SAFB通过调控RNA聚合酶I的活性参与核糖体RNA(rRNA)的合成，重组SAFB蛋白被用于体外结合实验，证实其与rDNA启动区的直接相互作用，影响rRNA转录效率。
2. **"SAFB promotes double-strand break repair through interaction with HDAC1 in BRCA1-deficient cells"**
- **作者**: Li, X. et al.
- **摘要**: 本文利用重组SAFB蛋白探究其在DNA损伤修复中的作用，发现SAFB通过结合组蛋白去乙酰化酶HDAC1.促进BRCA1缺陷细胞的同源重组修复，为乳腺癌治疗提供潜在靶点。
3. **"Recombinant SAFB protein inhibits estrogen receptor α-mediated transcription by competing with coactivators"**
- **作者**: Townson, S.M. et al.
- **摘要**: 研究通过表达纯化SAFB重组蛋白，证明其通过竞争性结合雌激素受体α(ERα)抑制转录活性，揭示了SAFB在激素信号通路中作为转录抑制因子的分子机制。
**Background of SAFB Recombinant Protein**
Scaffold Attachment Factor B (SAFB) is a nuclear protein belonging to the scaffold attachment factor (SAF) family, which includes SAFB1 and its paralog SAFB2. These proteins are implicated in chromatin organization, transcriptional regulation, and RNA processing. SAFB interacts with the nuclear matrix and functions as a molecular scaffold, mediating interactions between chromatin, transcription factors, and RNA polymerase II. It contains conserved RNA-recognition motifs (RRMs) and a glutamine-rich domain, enabling roles in RNA binding, splicing, and stress response pathways.
SAFB is involved in diverse cellular processes, including estrogen receptor (ER) signaling, DNA damage response, and apoptosis. Studies highlight its dual role as both a transcriptional repressor and co-activator, depending on cellular context. Dysregulation of SAFB has been linked to cancers, neurodegenerative diseases, and autoimmune disorders, underscoring its biological significance.
Recombinant SAFB proteins are engineered for *in vitro* and *in vivo* studies to dissect molecular mechanisms. Produced via bacterial (e.g., *E. coli*) or mammalian expression systems, these proteins retain functional domains for binding DNA, RNA, or partner proteins. Tags like His or GST facilitate purification and detection. Recombinant SAFB enables investigations into protein-protein/DNA/RNA interactions, structural analyses, and high-throughput screening for therapeutic targets. Its utility extends to modeling diseases linked to SAFB dysfunction and exploring its potential as a biomarker or drug target.
Overall, SAFB recombinant proteins serve as critical tools for unraveling its multifaceted roles in genome stability, gene expression, and disease pathogenesis.
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