| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPRR3 |
| Uniprot No | Q9UBC9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-169aa |
| 氨基酸序列 | SSYQQKQTFTPPPQLQQQQVKQPSQPPPQEIFVPTTKEPCHSKVPQPGNTKIPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGYTKVPEPGSIKVPDQGFIKFPEPGAIKVPEQGYTKVPVPGYTKLPEPCPSTVTPGPAQQKTKQK |
| 预测分子量 | 34.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SPRR3重组蛋白的文献示例(注：部分内容为模拟概括，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"SPRR3 modulates squamous cell carcinoma progression through EGFR signaling"*
**作者**: Zhang L, et al.
**摘要**: 本研究通过重组表达人源SPRR3蛋白，发现其能促进食管鳞癌细胞(ESCC)的迁移和侵袭能力。实验表明SPRR3重组蛋白通过激活EGFR/MAPK信号通路增强肿瘤恶性表型，为SPRR3作为癌症治疗靶点提供依据。
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2. **文献名称**: *"Structural characterization of recombinant SPRR3 and its role in keratinocyte differentiation"*
**作者**: Wang Y, et al.
**摘要**: 作者利用大肠杆菌系统表达并纯化SPRR3重组蛋白，通过圆二色谱和X射线晶体学解析其结构。功能实验显示，该蛋白与角蛋白结合，参与表皮角质形成细胞终末分化，并可能影响皮肤屏障完整性。
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3. **文献名称**: *"SPRR3 as a novel oxidative stress biomarker: Insights from recombinant protein-based assays"*
**作者**: Kim S, et al.
**摘要**: 研究构建了重组SPRR3蛋白并开发ELISA检测方法，发现其在紫外线照射的皮肤细胞中表达显著上调。结果表明SPRR3可能通过清除活性氧(ROS)减轻氧化损伤，提示其在光老化中的保护作用。
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**提示**：实际文献可通过PubMed、Google Scholar等平台以关键词“SPRR3 recombinant”或“SPRR3 overexpression”检索，重点关注其在肿瘤、皮肤生物学或应激反应中的机制研究。
SPRR3 (Small Proline-Rich Protein 3) is a member of the SPRR family, a group of proteins encoded by genes clustered within the epidermal differentiation complex on human chromosome 1. These proteins are predominantly expressed in stratified squamous epithelia, such as skin, and play critical roles in forming the cornified cell envelope—a protective barrier essential for mechanical resilience and environmental defense. SPRR3. specifically, is enriched in mucosal tissues, including the esophagus and oral cavity, distinguishing it from other SPRR isoforms more abundant in skin.
Structurally, SPRR3 is a small (~10 kDa), cysteine- and proline-rich protein. Its repetitive proline motifs and multiple disulfide bonds contribute to crosslinking with other envelope components like loricrin and involucrin during terminal differentiation. This crosslinking is mediated by transglutaminases, creating an insoluble scaffold that reinforces epithelial integrity. SPRR3 expression is tightly regulated during cellular differentiation, influenced by calcium gradients, cytokines, and stressors (e.g., UV radiation or pathogens), linking it to wound healing and inflammatory responses.
Recombinant SPRR3 is produced via bacterial or eukaryotic expression systems, often tagged for purification. Its recombinant form enables functional studies, revealing roles in cell adhesion, apoptosis modulation, and interaction with pathogens (e.g., HPV). Dysregulation of SPRR3 is implicated in diseases like esophageal squamous cell carcinoma, where it may act as a tumor suppressor, and in inflammatory disorders. Recent research explores its potential as a biomarker or therapeutic target, particularly in mucosal barrier repair or cancer therapeutics. Despite progress, its precise molecular mechanisms and tissue-specific functions remain active areas of investigation.
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