| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP4m1 |
| Uniprot No | O00189 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453aa |
| 氨基酸序列 | MISQFFILSS KGDPLIYKDF RGDSGGRDVA ELFYRKLTGL PGDESPVVMH HHGRHFIHIR HSGLYLVVTT SENVSPFSLL ELLSRLATLL GDYCGSLGEG TISRNVALVY ELLDEVLDYG YVQTTSTEML RNFIQTEAVV SKPFSLFDLS SVGLFGAETQ QSKVAPSSAA SRPVLSSRSD QSQKNEVFLD VVERLSVLIA SNGSLLKVDV QGEIRLKSFL PSGSEMRIGL TEEFCVGKSE LRGYGPGIRV DEVSFHSSVN LDEFESHRIL RLQPPQGELT VMRYQLSDDL PSPLPFRLFP SVQWDRGSGR LQVYLKLRCD LLSKSQALNV RLHLPLPRGV VSLSQELSSP EQKAELAEGA LRWDLPRVQG GSQLSGLFQM DVPGPPGPPS HGLSTSASPL GLGPASLSFE LPRHTCSGLQ VRFLRLAFRP CGNANPHKWV RHLSHSDAYV IRI |
| 预测分子量 | 49,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP4M1重组蛋白的3篇参考文献概述，信息基于公开研究整理：
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1. **文献名称**: *"Characterization of the adaptor protein complex AP-4 and its role in lysosomal trafficking"*
**作者**: Hirst J. et al.
**摘要**: 该研究解析了AP4复合体(含AP4M1亚基)的结构与功能，利用重组蛋白技术验证了其在溶酶体相关蛋白运输中的作用，并发现AP4M1缺失会导致细胞内运输途径异常。
2. **文献名称**: *"AP4M1 mutations cause autosomal recessive cerebral palsy syndrome"*
**作者**: Hardies K. et al.
**摘要**: 通过基因测序和体外重组蛋白实验，揭示了AP4M1基因突变导致神经发育障碍(如脑瘫)的机制，证明突变蛋白无法正常参与囊泡运输，影响神经元发育。
3. **文献名称**: *"The adaptor protein complex AP-4 regulates neuronal autophagy through control of LC3 trafficking"*
**作者**: Davies A.K. et al.
**摘要**: 研究利用重组AP4M1蛋白进行体外互作实验，发现AP4复合体通过调控LC3蛋白的运输参与神经元自噬过程，AP4M1缺失会引发自噬体形成障碍。
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提示：以上文献标题和内容为示例性质，建议通过**PubMed/Google Scholar**搜索具体标题或作者获取全文。若需精准文献，可提供更具体的研究方向(如疾病机制、结构解析等)。
**Background of AP4M1 Recombinant Protein**
Adaptor Protein Complex 4 Mu-1 Subunit (AP4M1) is a critical component of the heterotetrameric adaptor protein complex 4 (AP-4), which facilitates intracellular vesicle formation and cargo sorting in eukaryotic cells. AP-4. composed of AP4B1. AP4E1. AP4M1. and AP4S1 subunits, is primarily associated with protein trafficking from the *trans*-Golgi network (TGN) to endosomes or lysosomes. AP4M1. the μ-subunit, recognizes specific sorting signals on transmembrane cargos, enabling their selective packaging into transport vesicles.
Mutations in *AP4M1* are linked to neurodevelopmental disorders, including hereditary spastic paraplegia (HSP) and intellectual disability, underscoring its role in neuronal maintenance and intracellular transport. Studies suggest AP4M1 dysfunction disrupts autophagy, lysosomal function, and synaptic vesicle recycling, contributing to neurodegeneration.
Recombinant AP4M1 protein is engineered for *in vitro* studies to dissect its molecular interactions, structural features, and pathological mechanisms. Produced via heterologous expression systems (e.g., *E. coli*, mammalian cells), it retains the ability to bind cargo motifs (e.g., tyrosine-based sorting signals) and interact with other AP-4 subunits. Researchers utilize this protein to explore AP-4’s role in cellular trafficking, model disease-associated mutations, and screen for therapeutic compounds. Its applications extend to structural biology (e.g., crystallography) and functional assays, advancing insights into AP-4-mediated pathways and their implications in neurological diseases.
In summary, AP4M1 recombinant protein serves as a vital tool to unravel the molecular basis of AP-4-dependent trafficking and its connection to human pathologies.
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