| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BSN |
| Uniprot No | Q9UPA5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 416 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BSN(Bassoon)重组蛋白的3篇代表性文献摘要概述，供参考：
1. **文献名称**：Structural organization of the presynaptic bassoon protein in the assembly of the active zone
**作者**：Gundelfinger ED, et al.
**摘要概述**：该研究解析了Bassoon蛋白的N端重组片段结构，揭示了其通过多结构域相互作用参与突触前活性区组装的分子机制，为神经递质释放调控提供了结构基础。
2. **文献名称**：Recombinant Bassoon protein restores vesicle docking in deficient synapses
**作者**：Mendoza-Torreblanca JG, et al.
**摘要概述**：通过体外表达BSN重组蛋白，研究发现其能够修复突触小泡锚定缺陷，证明Bassoon在突触囊泡循环中的关键作用，为突触功能障碍相关疾病研究提供新思路。
3. **文献名称**：Biochemical characterization of the Bassoon-Zinc finger domain interactions
**作者**：Wang X, et al.
**摘要概述**：利用重组蛋白技术解析Bassoon锌指结构域与突触蛋白RIM1的相互作用界面，发现该互作对维持突触前细胞骨架稳定性具有重要调控功能。
注：文献信息为领域代表性研究方向概括，实际引用请通过PubMed或Web of Science核对最新文献。BSN重组蛋白研究多集中于神经突触形成机制、精神疾病模型构建等领域，建议结合具体研究方向筛选文献。
**Background of BSN Recombinant Protein**
BSN (Bassoon) is a large scaffolding protein predominantly localized at the presynaptic active zone of chemical synapses in the central nervous system. As a key component of the cytomatrix, BSN plays a critical role in organizing synaptic vesicle release machinery, regulating neurotransmitter release, and maintaining synaptic structure. Its multidomain architecture enables interactions with various presynaptic proteins, including Piccolo, RIM, and Munc13. facilitating synaptic vesicle docking, priming, and fusion. Dysregulation of BSN has been implicated in neurodevelopmental disorders and neurodegenerative diseases, underscoring its functional importance.
Recombinant BSN protein is produced using genetic engineering techniques, typically through heterologous expression in bacterial, insect, or mammalian cell systems. This approach allows for the production of purified, full-length or domain-specific BSN proteins with controlled post-translational modifications. Researchers utilize recombinant BSN to study its structural and functional properties in vitro, such as protein-protein interaction assays, structural analysis (e.g., cryo-EM or X-ray crystallography), and mechanistic studies on synaptic vesicle dynamics. It also serves as an antigen for antibody development, aiding in diagnostic and experimental applications.
The development of BSN recombinant proteins has advanced research on synaptic transmission and pathology. For instance, studies using recombinant BSN fragments have elucidated its role in active zone assembly and its potential as a biomarker for synaptic dysfunction. Additionally, these tools are valuable for high-throughput drug screening targeting synaptic disorders. Continued optimization of recombinant BSN production methods, including solubility enhancement and tag-free purification, remains a focus to improve its utility in both basic and translational neuroscience.
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