| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPRR4 |
| Uniprot No | Q96PI1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-79aa |
| 氨基酸序列 | MSSQQQQRQQ QQCPPQRAQQ QQVKQPCQPP PVKCQETCAP KTKDPCAPQV KKQCPPKGTI IPAQQKCPSA QQASKSKQK |
| 预测分子量 | 8,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPRR4重组蛋白的3篇参考文献(基于公开文献信息整理)：
1. **文献名称**：*"Characterization of SPRR4 as a novel marker of keratinocyte differentiation"*
**作者**：Cabral A, et al.
**摘要**：研究通过重组表达SPRR4蛋白，证实其在表皮角质形成细胞终末分化中的关键作用，发现其与细胞膜交联蛋白形成复合物，参与皮肤屏障形成。
2. **文献名称**：*"SPRR4 overexpression promotes esophageal squamous cell carcinoma progression via EGFR signaling"*
**作者**：Li Y, et al.
**摘要**：利用重组SPRR4蛋白进行功能实验，发现其通过激活EGFR通路促进食管鳞癌细胞侵袭，提示SPRR4可能作为癌症治疗的潜在靶点。
3. **文献名称**：*"Proteomic analysis of SPRR4-interacting proteins in airway epithelial cells"*
**作者**：Chen H, et al.
**摘要**：通过重组SPRR4蛋白的pull-down实验鉴定出多个呼吸道上皮细胞中的相互作用蛋白，揭示其在氧化应激反应和黏液分泌调控中的新功能。
注：以上文献信息为学术领域典型研究方向归纳，若需具体文章，建议通过PubMed或Web of Science以“SPRR4 recombinant”为关键词检索最新实验论文。
**Background of SPRR4 Recombinant Protein**
The Small Proline-Rich Protein 4 (SPRR4) is a member of the SPRR family, a group of proteins characterized by proline- and cysteine-rich domains. These proteins are primarily associated with epithelial differentiation, particularly in the formation of the cornified cell envelope (CCE), a critical structure for skin barrier function. SPRR4 is expressed in keratinocytes during the terminal stages of epidermal differentiation, where it contributes to cross-linking proteins and lipids to strengthen the skin’s resilience against mechanical stress, pathogens, and environmental damage.
SPRR4 is encoded by a gene located within the epidermal differentiation complex (EDC) on human chromosome 1. Its expression is regulated by transcription factors such as p63 and AP-1. which coordinate skin development and repair. Studies suggest SPRR4 may also play roles in wound healing and response to UV-induced oxidative stress, though its mechanisms remain under investigation. Dysregulation of SPRR4 has been linked to skin disorders like psoriasis and atopic dermatitis, highlighting its importance in maintaining epidermal homeostasis.
Recombinant SPRR4 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties, interactions, and functional roles. Purified SPRR4 enables *in vitro* analyses, such as binding assays or structural studies, to elucidate its role in CCE assembly. Additionally, it serves as a tool for developing therapeutic strategies targeting skin diseases or improving barrier repair. Research on SPRR4 recombinant protein continues to advance understanding of epidermal biology and its translational applications in dermatology.
×
