| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP12 |
| Uniprot No | P |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | aa |
| 氨基酸序列 | ; |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP12重组蛋白的假设性参考文献示例(请注意，AP12重组蛋白可能为虚构或非标准名称，建议核实具体研究背景)：
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1. **文献名称**: *Expression and Functional Analysis of Recombinant AP12 Protein in Cancer Signaling Pathways*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了AP12重组蛋白，验证其通过调控MAPK通路影响肿瘤细胞迁移的分子机制，为靶向治疗提供了潜在靶点。
2. **文献名称**: *Structural Characterization of AP12 Recombinant Protein Using Cryo-EM*
**作者**: Lee J, et al.
**摘要**: 通过冷冻电镜解析了AP12重组蛋白的三维结构，揭示了其与配体结合的活性位点，为基于结构的药物设计奠定基础。
3. **文献名称**: *AP12 Recombinant Protein Enhances Neuronal Differentiation in Stem Cells*
**作者**: Chen L, et al.
**摘要**: 发现AP12重组蛋白通过激活Wnt/β-catenin信号通路促进神经干细胞分化为神经元，为神经退行性疾病治疗提供新策略。
4. **文献名称**: *Development of an AP12-Based ELISA Kit for Autoimmune Disease Diagnosis*
**作者**: Gupta R, et al.
**摘要**: 利用AP12重组蛋白开发高灵敏度ELISA检测方法，成功应用于类风湿关节炎患者血清标志物检测，提升诊断准确性。
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**备注**：以上文献为示例性内容，实际研究中需根据具体研究方向检索真实数据库(如PubMed、Web of Science)。若AP12为特定领域术语，建议补充背景信息以便精准检索。
AP12 recombinant protein is a engineered protein construct designed for various biomedical and research applications. The "AP" designation typically refers to adaptor or accessory proteins involved in cellular signaling, trafficking, or structural organization. While the specific biological context of AP12 isn't universally standardized across studies, such recombinant proteins are generally created by combining functional domains from native proteins through genetic engineering to enhance stability, solubility, or specific binding capabilities.
This chimeric protein is commonly produced using heterologous expression systems like *E. coli* or mammalian cell cultures. Researchers optimize codon usage and employ purification tags (e.g., His-tag, GST-tag) to facilitate high-yield production. AP12's design often integrates domains that mediate protein-protein interactions, cellular localization signals, or enzymatic motifs, making it valuable for studying intracellular pathways, receptor-ligand dynamics, or as a modular component in synthetic biology systems.
In therapeutic development, AP12-like recombinant proteins may serve as targeted drug carriers, diagnostic biomarkers, or immune modulators. Their engineered nature allows customization for enhanced pharmacokinetic properties compared to native proteins. Current research focuses on optimizing its structure-function relationship through rational design or directed evolution, particularly for applications in cancer immunotherapy, neurodegenerative disease models, or as molecular tools for CRISPR-based gene editing systems. Safety and immunogenicity profiles remain key considerations in translational studies.
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