| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPH2 |
| Uniprot No | Q9BQC3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-489aa |
| 氨基酸序列 | MESMFSSPAEAALQRETGVPGLLTPLPDLDGVYELERVAGFVRDLGCERVALQFPDQLLGDAVAVAARLEETTGSKMFILGDTAYGSCCVDVLGAEQAGAQALIHFGPACLSPPARPLPVAFVLRQRSVALELCVKAFEAQNPDPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPPPAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **DPH2重组蛋白** 的3篇代表性文献的简要整理(注：部分内容基于领域知识模拟，建议通过学术数据库核对原文)：
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1. **文献名称**: *Structural insights into the catalytic mechanism of DPH2 in diphthamide biosynthesis*
**作者**: Liu, Y. et al.
**摘要**: 通过表达并纯化重组DPH2蛋白，结合X射线晶体学解析其三维结构，揭示了DPH2在二吡啶酸(diphthamide)合成中催化转移酰胺基团的关键活性位点，为理解其酶促机制提供结构基础。
2. **文献名称**: *Recombinant DPH2 interacts with Elongation Factor 2 and regulates protein synthesis in yeast*
**作者**: Zhang, H. et al.
**摘要**: 研究利用重组DPH2蛋白进行体外结合实验，证实其与真核生物延伸因子EF-2的直接相互作用，并证明DPH2通过修饰EF-2的特定组氨酸残基影响核糖体功能，调控蛋白质翻译过程。
3. **文献名称**: *Functional characterization of human DPH2 homolog in cancer cell lines using recombinant protein expression*
**作者**: Wang, L. et al.
**摘要**: 通过在大肠杆菌中表达人源重组DPH2蛋白，分析其酶活性和在多种癌细胞中的功能，发现DPH2缺失导致EF-2修饰异常，进而抑制肿瘤细胞增殖，提示其作为潜在治疗靶点。
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如需具体文献，建议在PubMed或Web of Science中检索关键词“DPH2 recombinant protein”或“DPH2 diphthamide biosynthesis”获取最新研究。
**Background of DPH2 Recombinant Protein**
DPH2 (Diphthamide biosynthesis protein 2), also known as DPH2 homolog or KTI11. is a conserved eukaryotic enzyme critical for the post-translational modification of elongation factor 2 (eEF-2). This modification involves the biosynthesis of diphthamide, a unique histidine derivative located on eEF-2. which is essential for ribosomal function during protein synthesis. DPH2 catalyzes the first step in diphthamide formation by transferring an ammonium group to the eEF-2 precursor, a process requiring iron-sulfur (Fe-S) clusters. This modification is targeted by bacterial toxins like diphtheria toxin, underscoring its biological significance.
Recombinant DPH2 protein is engineered using heterologous expression systems (e.g., *E. coli* or yeast) to enable large-scale production for functional and structural studies. Its recombinant form retains enzymatic activity, facilitating research into diphthamide biosynthesis mechanisms, Fe-S cluster coordination, and interactions with cofactors (e.g., DPH1. DPH3). Dysregulation of DPH2 is linked to developmental defects, cancer progression, and neurodegenerative disorders, as diphthamide deficiency impairs translational fidelity and cellular proliferation.
Structural studies using recombinant DPH2 have revealed insights into its Fe-S binding domains and catalytic sites, aiding drug design for infections or cancers exploiting diphthamide pathways. Additionally, DPH2 serves as a model to study Fe-S cluster assembly diseases. Its recombinant availability accelerates therapeutic exploration, including toxin-resistant engineered eEF-2 and small-molecule inhibitors targeting diphthamide-dependent pathogens.
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