| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEL |
| Uniprot No | P19835 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-753aa |
| 氨基酸序列 | AKLGAVYTEG GFVEGVNKKL GLLGDSVDIF KGIPFAAPTK ALENPQPHPG WQGTLKAKNF KKRCLQATIT QDSTYGDEDC LYLNIWVPQG RKQVSRDLPV MIWIYGGAFL MGSGHGANFL NNYLYDGEEI ATRGNVIVVT FNYRVGPLGF LSTGDANLPG NYGLRDQHMA IAWVKRNIAA FGGDPNNITL FGESAGGASV SLQTLSPYNK GLIRRAISQS GVALSPWVIQ KNPLFWAKKV AEKVGCPVGD AARMAQCLKV TDPRALTLAY KVPLAGLEYP MLHYVGFVPV IDGDFIPADP INLYANAADI DYIAGTNNMD GHIFASIDMP AINKGNKKVT EEDFYKLVSE FTITKGLRGA KTTFDVYTES WAQDPSQENK KKTVVDFETD VLFLVPTEIA LAQHRANAKS AKTYAYLFSH PSRMPVYPKW VGADHADDIQ YVFGKPFATP TGYRPQDRTV SKAMIAYWTN FAKTGDPNMG DSAVPTHWEP YTTENSGYLE ITKKMGSSSM KRSLRTNFLR YWTLTYLALP TVTDQEATPV PPTGDSEATP VPPTGDSETA PVPPTGDSGA PPVPPTGDSG APPVPPTGDS GAPPVPPTGD SGAPPVPPTG DSGAPPVPPT GDSGAPPVPP TGDSGAPPVP PTGDSGAPPV PPTGDAGPPP VPPTGDSGAP PVPPTGDSGA PPVTPTGDSE TAPVPPTGDS GAPPVPPTGD SEAAPVPPTD DSKEAQMPAV IRF |
| 预测分子量 | 79,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CEL(羧酸酯酶)重组蛋白研究的文献示例，包含标题、作者及摘要概括：
1. **标题**：*"High-level expression and characterization of a novel recombinant human carboxylesterase 1 (rhCE1) in Pichia pastoris"*
**作者**：Li Y, et al.
**摘要**：研究报道了在毕赤酵母系统中高效表达重组人羧酸酯酶1(rhCE1)，通过优化密码子和发酵条件显著提升酶活性，并验证其在药物前体水解中的应用潜力。
2. **标题**：*"Functional characterization of recombinant carboxylesterase from Pseudomonas aeruginosa for biodegradation of plasticizers"*
**作者**：Wang X, et al.
**摘要**：该研究克隆并表达了铜绿假单胞菌来源的CEL重组蛋白，证实其对邻苯二甲酸酯类增塑剂的高效降解能力，为环境污染修复提供新策略。
3. **标题**：*"Structural and kinetic analysis of a recombinant carboxylesterase from marine microbial metagenome"*
**作者**：Kim S, et al.
**摘要**：通过宏基因组学筛选得到海洋微生物CEL基因，重组表达后解析其三维结构，揭示其低温高催化活性的分子机制，拓展工业酶应用场景。
注：上述文献为模拟示例，实际引用时需核对真实数据库(如PubMed、Web of Science)获取准确信息。
CEL recombinant protein, derived from the carboxyl ester lipase (CEL) gene, is a key focus in metabolic and pancreatic disease research. CEL, also known as bile salt-dependent carboxyl ester hydrolase or cholesterol esterase, is a digestive enzyme primarily synthesized in the pancreatic acinar cells and secreted into the intestinal lumen. It plays a critical role in hydrolyzing dietary cholesterol esters, lipid-soluble vitamins, and other esterified substrates, contributing to lipid absorption and metabolism. The CEL gene’s polymorphisms and mutations have been linked to pancreatic disorders (e.g., chronic pancreatitis) and diabetes (e.g., maturity-onset diabetes of the young type 8. MODY8), driving interest in its recombinant form for functional studies.
Recombinant CEL is produced using genetic engineering techniques, often expressed in microbial (e.g., *E. coli*) or mammalian cell systems to ensure proper post-translational modifications. This allows large-scale production of highly pure, bioactive protein for research and therapeutic applications. Its applications span enzymology, drug discovery, and disease mechanism studies. For instance, recombinant CEL aids in elucidating how specific mutations disrupt enzyme activity or promote pathogenic protein aggregation in pancreatic tissue. It also serves as a tool for screening enzyme inhibitors or stabilizers for metabolic disease therapy.
Additionally, recombinant CEL is explored as a biologics candidate for enzyme replacement in pancreatic insufficiency and as an antigen in autoimmune diabetes research. Its pathogen-free production and scalability make it advantageous over animal-derived counterparts. Recent studies also investigate its role in lipid-mediated signaling and potential use in drug delivery systems. Overall, CEL recombinant protein bridges basic science and translational medicine, offering insights into digestive disorders, diabetes pathogenesis, and therapeutic innovation.
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