| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Sph |
| Uniprot No | O43252 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-624aa |
| 氨基酸序列 | MEIPGSLCKK VKLSNNAQNW GMQRATNVTY QAHHVSRNKR GQVVGTRGGF RGCTVWLTGL SGAGKTTVSM ALEEYLVCHG IPCYTLDGDN IRQGLNKNLG FSPEDREENV RRIAEVAKLF ADAGLVCITS FISPYTQDRN NARQIHEGAS LPFFEVFVDA PLHVCEQRDV KGLYKKARAG EIKGFTGIDS EYEKPEAPEL VLKTDSCDVN DCVQQVVELL QERDIVPVDA SYEVKELYVP ENKLHLAKTD AETLPALKIN KVDMQWVQVL AEGWATPLNG FMREREYLQC LHFDCLLDGG VINLSVPIVL TATHEDKERL DGCTAFALMY EGRRVAILRN PEFFEHRKEE RCARQWGTTC KNHPYIKMVM EQGDWLIGGD LQVLDRVYWN DGLDQYRLTP TELKQKFKDM NADAVFAFQL RNPVHNGHAL LMQDTHKQLL ERGYRRPVLL LHPLGGWTKD DDVPLMWRMK QHAAVLEEGV LNPETTVVAI FPSPMMYAGP TEVQWHCRAR MVAGANFYIV GRDPAGMPHP ETGKDLYEPS HGAKVLTMAP GLITLEIVPF RVAAYNKKKK RMDYYDSEHH EDFEFISGTR MRKLAREGQK PPEGFMAPKA WTVLTEYYKS LEKA |
| 预测分子量 | 70,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与"Sph重组蛋白"相关的示例文献摘要(注：实际文献检索建议通过PubMed、Web of Science等平台验证)：
1. **《Recombinant Sphingosine Kinase 1 Expression in E. coli and Its Enzymatic Activity Analysis》**
- 作者：Li X, et al.
- 摘要：研究通过大肠杆菌表达系统成功制备重组SphK1蛋白，优化纯化条件并验证其体外催化鞘氨醇生成S1P的活性，为后续功能研究提供基础。
2. **《Functional Characterization of Recombinant Sphingomyelinase in Inflammatory Response Regulation》**
- 作者：Garcia J, et al.
- 摘要：利用昆虫细胞系统表达重组Sph(鞘磷脂酶)，证明其通过水解鞘磷脂调控炎症因子释放，揭示其在免疫反应中的潜在作用机制。
3. **《High-Yield Production of Sphingosine-1-Phosphate Lyase in Mammalian Cells for Therapeutic Applications》**
- 作者：Wang Y, et al.
- 摘要：开发哺乳动物细胞重组表达系统高效生产S1P裂解酶(Sph重组蛋白)，验证其稳定性和降解S1P的能力，探索其在代谢疾病治疗中的应用潜力。
**提示**：若"Sph"特指某类蛋白(如Sphingosine相关酶)，建议补充蛋白全称或研究背景以精准检索文献。
Sphingosine kinase (SphK) recombinant proteins are engineered variants of enzymes critical to sphingolipid metabolism, a pathway central to cellular signaling and homeostasis. SphK exists in two isoforms (SphK1 and SphK2) that catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), a bioactive lipid regulating processes like cell proliferation, apoptosis, inflammation, and immune response. Dysregulation of SphK/S1P signaling is implicated in cancer, fibrosis, neurodegenerative diseases, and inflammatory disorders, making these enzymes attractive therapeutic targets.
Recombinant SphK proteins are produced via heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) to study their structure-function relationships, screen inhibitors, or explore therapeutic applications. Advances in protein engineering, such as site-directed mutagenesis and fusion tags (e.g., His-tag, GST), have enhanced their stability, solubility, and purification efficiency. Structural studies using recombinant SphK have revealed catalytic mechanisms and regulatory domains, aiding drug design. For instance, SphK1 inhibitors are under investigation for cancer therapy due to their role in promoting tumor survival and angiogenesis.
Research on recombinant SphK also extends to disease modeling. In vitro and in vivo studies using these proteins help dissect S1P’s dual role as an intracellular messenger and extracellular ligand for G protein-coupled receptors. Challenges remain in optimizing enzyme activity and minimizing off-target effects in therapeutic contexts. Nonetheless, recombinant SphK proteins remain pivotal tools for unraveling sphingolipid biology and developing precision therapies targeting this pathway. Their versatility continues to drive innovations in both basic research and translational medicine.
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