| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHa8 |
| Uniprot No | Q9Y5H6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-950aa |
| 氨基酸序列 | MDYHWRGELGSWRLLLLLLLLAAWKVGSGQLHYSVPEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKRHRDLLEVSLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVDVEVKDVNDNPPVFRVKDQKLFVSESRMPDSRFPLEGASDADVGANSVLTYRLSSHDYFMLDVNSKNDENKLVELVLRKSLDREDAPAHHLFLTATDGGKPELTGTVQLLVTVLDVNDNAPTFEQSEYEVRIFENADNGTTVIKLNASDPDEGANGAISYSFNSLVETMVIDHFSIDRNTGEIVIRGNLDFEQENLYKILIDATDKGHPPMAGHCTVLVRILDKNDNVPEIALTSLSLPVREDAQFGTVIALISVNDLDSGANGQVTCSLMPHVPFKLVSTFKNYYSLVLDSALDRERVSAYELVVTARDGGSPSLWATASLSVEVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSARDADAQENALVSYSLVERRVGERSLSSYISVHTESGKVYALQPLDHEELELLQFQVSARDAGVPPLGSNVTLQVFVLDENDNAPALLEPRVGGTGGAASKLVPRSVGAGHVVAKVRAVDADSGYNAWLSYELQPAASSPRIPFRVGLYTGEISTTRVLDEADSPRHRLLVLVKDHGEPALTATATVLVSLVESGQAPKASSRQSAGVLGPEAALVDVNVYLIIAICAVSSLLVLTLLLYTALRCSALPTEGGCRAGKPTLVCSSAVGSWSYSQQQPQRVCSGEGPPKTDLMAFSPCLPPDLGSVDVGEEQDLNVDHGLKPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ |
| 预测分子量 | 103 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHα8重组蛋白的虚构参考文献示例(仅供格式参考，非真实文献)：
1. **《Recombinant expression and functional characterization of PCDHα8 in neural development》**
- **作者**: Li, X., et al.
- **摘要**: 本研究在大肠杆菌中成功表达并纯化了PCDHα8重组蛋白，验证其在体外促进神经元突触形成的功能，揭示了其通过钙依赖性信号通路调控神经细胞黏附的机制。
2. **《Structural insights into PCDHα8 extracellular domain by X-ray crystallography》**
- **作者**: Chen, Y., & Wang, J.
- **摘要**: 通过重组表达人源PCDHα8的胞外结构域并解析其晶体结构，发现其独特的钙离子结合位点，为理解原钙粘蛋白家族的结构-功能关系提供依据。
3. **《PCDHα8 knockdown and recombinant rescue in autism-related behavioral models》**
- **作者**: Smith, R.L., et al.
- **摘要**: 利用重组PCDHα8蛋白在小鼠模型中逆转自闭症相关社交行为缺陷，证明其在大脑皮层发育中的关键作用及潜在治疗应用价值。
4. **《Optimization of PCDHα8 recombinant protein production in mammalian cells》**
- **作者**: Kim, H., et al.
- **摘要**: 开发了一种基于HEK293细胞的高效PCDHα8重组蛋白表达系统，验证其糖基化修饰对蛋白稳定性和细胞膜定位的影响。
注：以上文献为模拟内容，实际研究中请通过PubMed/Google Scholar等平台检索真实文献。
**Background of PCDHα8 Recombinant Protein**
Protocadherins (PCDHs) are a subgroup of the cadherin superfamily, known for their roles in cell-cell adhesion and neural development. Among them, the clustered protocadherins (PCDH-α, -β, and -γ) are predominantly expressed in the brain and implicated in neuronal connectivity, synaptic plasticity, and self-avoidance of dendrites. PCDHα8. a member of the PCDH-α subfamily, is characterized by its extracellular cadherin repeat domains, a transmembrane region, and a variable cytoplasmic tail that distinguishes it from non-clustered protocadherins.
PCDHα8 plays a critical role in establishing neural circuit specificity by mediating selective interactions between neurons. Its expression is regulated by promoter choice and combinatorial splicing, contributing to the diversity of neuronal surface identities. Studies suggest that PCDHα8 is involved in axon guidance, dendritic self-avoidance, and synapse formation, with disruptions linked to neurodevelopmental disorders such as autism spectrum disorders (ASD) and schizophrenia.
Recombinant PCDHα8 protein is engineered to facilitate functional studies. Produced via heterologous expression systems (e.g., mammalian or insect cells), it retains the extracellular domain responsible for homophilic binding. This protein is often purified with affinity tags (e.g., Fc or His-tags) for applications like ligand-receptor interaction assays, structural studies (e.g., crystallography), or antibody generation. Researchers use it to dissect PCDHα8’s role in neural development, model pathological mechanisms, or screen therapeutic compounds targeting PCDH-related disorders.
Overall, PCDHα8 recombinant protein serves as a vital tool for unraveling the molecular basis of neural wiring and advancing translational research in neurodevelopmental diseases.
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