| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAGEB16 |
| Uniprot No | A2A368 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-324aa |
| 氨基酸序列 | MSQDQESPRC THDQHLQTFS ETQSLEVAQV SKALEKTLLS SSHPLVPGKL KEAPAAKAES PLEVPQSFCS SSIAVTTTSS SESDEASSNQ EEEDSPSSSE DTSDPRNVPA DALDQKVAFL VNFMLHKCQM KKPITKADML KIIIKDDESH FSEILLRASE HLEMIFGLDV VEVDPTTHCY GLFIKLGLTY DGMLSGEKGV PKTGLLIIVL GVIFMKGNRA TEEEVWEVLN LTGVYSGKKH FIFGEPRMLI TKDFVKEKYL EYQQVANSDP ARYEFLWGPR AKAETSKMKV LEFVAKVHGS YPHSFPSQYA EALKEEEERA RARI |
| 预测分子量 | 36,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAGEB16重组蛋白的3篇示例参考文献(请注意，以下内容为假设性示例，实际文献需通过学术数据库验证)：
---
1. **文献名称**: *Expression and Purification of Recombinant MAGEB16 Protein in E. coli*
**作者**: Zhang L, et al.
**摘要**: 本研究报道了通过大肠杆菌表达系统高效制备MAGEB16重组蛋白的方法，优化了诱导条件及纯化步骤，并通过Western blot验证其抗原性，为后续癌症免疫治疗研究提供基础材料。
2. **文献名称**: *MAGEB16 Promotes Tumor Cell Proliferation via PI3K/AKT Pathway*
**作者**: Kim S, et al.
**摘要**: 研究发现MAGEB16在多种癌症中高表达，通过重组蛋白体外实验证明其激活PI3K/AKT信号通路，促进肿瘤细胞增殖，提示其作为潜在治疗靶点的可能性。
3. **文献名称**: *Structural Characterization of MAGEB16 and Its Interaction with HLA Complex*
**作者**: Müller R, et al.
**摘要**: 利用重组MAGEB16蛋白进行X射线晶体学分析，解析其三维结构，并揭示其与人类白细胞抗原(HLA)的结合模式，为设计靶向MAGEB16的免疫疗法提供结构依据。
---
**注意事项**：
- 上述文献为示例，实际研究需通过PubMed、Google Scholar等平台检索。
- 推荐使用关键词："MAGEB16 recombinant protein"、"MAGEB16 cancer antigen" 或结合具体研究领域筛选文献。
- 可访问DOI数据库或联系作者获取全文。
**Background of MAGEB16 Recombinant Protein**
MAGEB16 (Melanoma-Associated Antigen B16) belongs to the MAGE protein family, a group of cancer-testis antigens (CTAs) predominantly expressed in germline cells and aberrantly reactivated in various cancers. These proteins share a conserved MAGE homology domain (MHD), which facilitates protein-protein interactions, often involved in modulating ubiquitin ligase complexes or transcriptional regulation. MAGEB16. specifically, is encoded by a gene located on the X chromosome and is normally restricted to immune-privileged tissues like the testes. However, its ectopic expression has been observed in malignancies such as melanoma, lung cancer, and glioblastoma, linking it to tumor progression, immune evasion, or therapy resistance.
Recombinant MAGEB16 refers to the artificially engineered version of the protein produced in heterologous systems (e.g., *E. coli* or mammalian cells) for research and therapeutic applications. Its production typically involves cloning the MAGEB16 gene into expression vectors, followed by purification using affinity chromatography. This recombinant protein retains functional domains, enabling studies on its biochemical properties, interaction partners (e.g., E3 ubiquitin ligases), and role in cancer biology.
Interest in MAGEB16 stems from its potential as a biomarker or immunotherapy target. As a CTA, it may elicit immune responses when expressed in tumors, making it a candidate for cancer vaccines or T-cell therapies. However, challenges include understanding its precise mechanistic contributions to oncogenesis and addressing its restricted expression patterns. Recombinant MAGEB16 serves as a critical tool for structural analysis, antibody development, and screening small-molecule inhibitors. Ongoing research aims to unravel its dual role in normal physiology and cancer, paving the way for targeted therapeutic strategies.
×
