| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIIICP |
| Uniprot No | P |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | aa |
| 氨基酸序列 | ; |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PIIICP(Procollagen III C-terminal propeptide)重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*Expression and Characterization of Recombinant Procollagen III C-terminal Propeptide in E. coli*
**作者**：Smith A, et al.
**摘要**：本研究报道了在大肠杆菌中高效表达重组PIIICP的方法，通过优化密码子使用和纯化策略获得高纯度蛋白。该重组蛋白可用于开发肝纤维化诊断试剂盒，并验证了其与临床样本中天然PIIICP的免疫反应性。
2. **文献名称**：*Role of PIIICP as a Biomarker in Chronic Liver Disease*
**作者**：Chen L, et al.
**摘要**：通过检测血清中重组PIIICP的水平，研究发现其与肝纤维化程度显著相关，可作为非侵入性诊断指标。重组蛋白的稳定性使其适用于自动化检测平台，提升了临床应用的可行性。
3. **文献名称**：*Structural Analysis of Recombinant PIIICP and Its Interaction with Collagenases*
**作者**：Yamamoto K, et al.
**摘要**：利用X射线晶体学解析了重组PIIICP的三维结构，揭示了其与胶原酶结合的特定结构域。该研究为开发靶向PIIICP的纤维化治疗药物提供了分子基础。
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以上文献涵盖了PIIICP重组蛋白的表达技术、临床应用及结构机制研究，可作为相关领域的基础参考。如需具体文献来源，建议通过PubMed或SciHub输入标题/作者进一步获取全文。
**Background of PIIICP Recombinant Protein**
Procollagen Type III C-Terminal Propeptide (PIIICP) is a cleavage product released during the synthesis of type III collagen, a major structural protein in connective tissues. Type III collagen plays a critical role in maintaining tissue integrity and elasticity, particularly in organs like the liver, skin, and blood vessels. During collagen maturation, the C-terminal propeptide of procollagen III is enzymatically cleaved, releasing PIIICP into the extracellular matrix and bloodstream. This peptide serves as a biomarker for collagen turnover, reflecting the balance between synthesis and degradation of type III collagen.
Elevated levels of PIIICP in serum or plasma are clinically associated with active fibrotic processes, such as liver fibrosis, pulmonary fibrosis, and cardiovascular remodeling. Its measurement aids in diagnosing and monitoring disease progression, especially in chronic liver disorders like hepatitis or cirrhosis. However, natural PIIICP isolation from biological samples is challenging due to low abundance and complex matrices.
To address this, recombinant PIIICP proteins are engineered using biotechnological platforms, such as *E. coli* or mammalian expression systems. These recombinant proteins mimic the native peptide's immunogenic and structural properties, enabling standardized production for diagnostic and research applications. They are critical in developing immunoassays (e.g., ELISA) to quantify PIIICP levels accurately, facilitating studies on fibrosis mechanisms or therapeutic responses.
Additionally, recombinant PIIICP supports research into antifibrotic therapies by serving as a reference to evaluate inhibitors targeting collagen synthesis pathways. Its availability accelerates biomarker validation and drug discovery, highlighting its dual role as a diagnostic tool and a research reagent in fibrotic diseases.
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