| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BSA |
| Uniprot No | O95870 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-558aa |
| 氨基酸序列 | MAKLLSCVLGPRLYKIYRERDSERAPASVPETPTAVTAPHSSSWDTYYQPRALEKHADSILALASVFWSISYYSSPFAFFYLYRKGYLSLSKVVPFSHYAGTLLLLLAGVACLRGIGRWTNPQYRQFITILEATHRNQSSENKRQLANYNFDFRSWPVDFHWEEPSSRKESRGGPSRRGVALLRPEPLHRGTADTLLNRVKKLPCQITSYLVAHTLGRRMLYPGSVYLLQKALMPVLLQGQARLVEECNGRRAKLLACDGNEIDTMFVDRRGTAEPQGQKLVICCEGNAGFYEVGCVSTPLEAGYSVLGWNHPGFAGSTGVPFPQNEANAMDVVVQFAIHRLGFQPQDIIIYAWSIGGFTATWAAMSYPDVSAMILDASFDDLVPLALKVMPDSWRGLVTRTVRQHLNLNNAEQLCRYQGPVLLIRRTKDEIITTTVPEDIMSNRGNDLLLKLLQHRYPRVMAEEGLRVVRQWLEASSQLEEASIYSRWEVEEDWCLSVLRSYQAEHGPDFPWSVGEDMSADGRRQLALFLARKHLHNFEATHCTPLPAQNFQMPWHL |
| 预测分子量 | 63,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组BSA蛋白的参考文献示例(注：以下为模拟示例，实际文献需通过学术数据库查询)：
1. **标题**: "High-yield production of recombinant bovine serum albumin in *E. coli*: Optimization and characterization"
**作者**: Smith A, et al.
**摘要**: 研究通过优化大肠杆菌表达系统提高重组BSA的产量，并验证其理化性质与传统BSA的一致性，为生物制药提供经济高效的替代方案。
2. **标题**: "Recombinant BSA as a stabilizer in vaccine formulations: Efficacy and safety assessment"
**作者**: Zhang Y, et al.
**摘要**: 评估重组BSA作为疫苗保护剂的性能，证实其在维持抗原稳定性方面的效果，且无动物源成分风险，适用于人用疫苗生产。
3. **标题**: "Comparative analysis of recombinant BSA production in yeast and mammalian cell systems"
**作者**: Lee J, et al.
**摘要**: 对比酵母与哺乳动物细胞表达重组BSA的差异，发现毕赤酵母系统可实现无糖基化修饰的高纯度蛋白，适用于细胞培养领域。
4. **标题**: "Advances in recombinant albumin: From bioprocessing to clinical applications"
**作者**: Johnson R, et al.
**摘要**: 综述重组白蛋白(包括BSA)的最新生产技术、纯化策略及在药物递送、诊断试剂等领域的应用潜力。
建议通过 **PubMed** 或 **Google Scholar** 检索关键词 "*recombinant BSA protein*" 或 "*recombinant bovine serum albumin*" 获取具体文献。
**Background of Recombinant BSA Protein**
Bovine serum albumin (BSA), a globular protein derived from bovine blood, has been widely utilized in biomedical research, diagnostics, and industrial applications due to its stability, solubility, and versatility. Traditionally, BSA is purified from bovine serum, but concerns over batch-to-batch variability, ethical issues, and potential contamination with pathogens (e.g., prions or viruses) have driven the development of recombinant BSA (rBSA) as a safer, more consistent alternative.
Recombinant BSA is produced using genetically engineered host systems, such as bacteria (e.g., *E. coli*), yeast, or mammalian cells. These systems express the BSA gene cloned from bovine sources, enabling large-scale production under controlled conditions. Advanced purification techniques, including affinity chromatography, ensure high purity (>98%) and reduced endotoxin levels, making rBSA suitable for sensitive applications like cell culture, molecular biology, and therapeutics.
A key advantage of rBSA lies in its reproducibility and customization. Unlike animal-derived BSA, recombinant versions eliminate risks of zoonotic contaminants and align with regulatory requirements for biologics and therapeutics. Additionally, engineered modifications (e.g., tag-free variants, site-specific mutations) allow tailoring for specific uses, such as enzyme stabilization, immunoassays, or nanoparticle coating.
In research, rBSA serves as a standard protein in quantitative assays (e.g., Bradford assay), a blocking agent in immunoassays, and a stabilizer for enzymes or antibodies. In biomanufacturing, it is employed as an excipient in vaccine formulations and cell culture media. Despite its benefits, challenges remain, including higher production costs and the need for optimization in expression systems to match native BSA’s post-translational modifications.
Overall, recombinant BSA represents a critical advancement in protein biotechnology, addressing both scientific and safety demands while expanding applications in modern life sciences and medicine.
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