| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABPC |
| Uniprot No | P13466 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-857aa |
| 氨基酸序列 | MAAAPSGKTWIDVQKKTFTGWANNYLKERILKIEDLATSLEDGVLLINLLEIISSKKILKYNKAPKIRMQKIENNNMAVNFIKSEGLKLVGIGAEDIVDSQLKLILGLIWTLILRYQIQMSESDNSPKAALLEWVRKQVAPYKVVVNNFTDSWCDGRVLSALTDSLKPGVREMSTLTGDAVQDIDRSMDIALEEYEIPKIMDANDMNSLPDELSVITYVSYFRDYALNKEKRDADALAALEKKRRETSDASKVEVYGPGVEGGFVNKSADFHIKAVNYYGEPLANGGEGFTVSVVGADGVEVPCKLVDNKNGIYDASYTATVPQDYTVVVQLDDVHCKDSPYNVKIDGSDAQHSNAYGPGLEGGKVGVPAAFKIQGRNKDGETVTQGGDDFTVKVQSPEGPVDAQIKDNGDGSYDVEYKPTKGGDHTVEVFLRGEPLAQGPTEVKILNSDSQNSYCDGPGFEKAQAKRPTEFTIHSVGADNKPCAAGGDPFQVSISGPHPVNVGITDNDDGTYTVAYTPEQPGDYEIQVTLNDEAIKDIPKSIHIKPAADPEKSYAEGPGLDGGECFQPSKFKIHAVDPDGVHRTDGGDGFVVTIEGPAPVDPVMVDNGDGTYDVEFEPKEAGDYVINLTLDGDNVNGFPKTVTVKPAPSAEHSYAEGEGLVKVFDNAPAEFTIFAVDTKGVARTDGGDPFEVAINGPDGLVVDAKVTDNNDGTYGVVYDAPVEGNYNVNVTLRGNPIKNMPIDVKCIEGANGEDSSFGSFTFTVAAKNKKGEVKTYGGDKFEVSITGPAEEITLDAIDNQDGTYTAAYSLVGNGRFSTGVKLNGKHIEGSPFKQVLGNPGKKNPEVKSFTTTRTAN |
| 预测分子量 | 92,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABPC(假设为氨苄青霉素相关重组蛋白)的示例参考文献，内容为虚构归纳，仅供参考格式：
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1. **文献名称**：Expression and Purification of Recombinant ABPC Protein in *E. coli*
**作者**：Zhang L, et al.
**摘要**：本研究利用大肠杆菌表达系统成功表达了带有His标签的ABPC重组蛋白，通过镍柱亲和层析纯化获得高纯度蛋白，并通过Western blot验证其特异性，为后续功能研究奠定基础。
2. **文献名称**：Structural Analysis of ABPC-binding Protein and Its Role in Antibiotic Resistance
**作者**：Smith J, Patel R.
**摘要**：通过X射线晶体学解析ABPC重组蛋白的三维结构，发现其活性中心与β-内酰胺类抗生素结合的关键位点，揭示了该蛋白介导细菌耐药性的分子机制。
3. **文献名称**：Development of an ABPC-Specific Biosensor Using Recombinant Protein Technology
**作者**：Chen H, et al.
**摘要**：将ABPC重组蛋白固定于纳米电极表面，构建高灵敏度生物传感器，可快速检测环境中氨苄青霉素残留，检测限低至0.1 ppm。
4. **文献名称**：Optimization of ABPC Recombinant Protein Production in Pichia pastoris
**作者**：Kim S, Lee W.
**摘要**：在毕赤酵母表达系统中优化ABPC重组蛋白的发酵条件(如pH、甲醇诱导浓度)，使蛋白产量提升3倍，并证实其酶活性优于原核表达系统产物。
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注：以上文献为示例性内容，实际引用需根据具体研究方向检索真实数据库(如PubMed、ScienceDirect)。
**Background of ABPC Recombinant Protein**
Recombinant protein technology has revolutionized biomedical research and therapeutic development by enabling the production of tailored proteins in controlled host systems. ABPC recombinant protein, a engineered variant derived from *[specific source or protein name, if applicable]*, exemplifies this advancement. Typically produced using expression systems like *E. coli*, yeast, or mammalian cells, recombinant proteins such as ABPC are designed to retain specific functional domains while optimizing stability, solubility, and purity for downstream applications.
The development of ABPC leverages genetic engineering to modify or enhance native protein properties, such as binding affinity, enzymatic activity, or resistance to degradation. For instance, ABPC may incorporate tags (e.g., His-tag) for simplified purification or mutations to eliminate undesired post-translational modifications. This precision ensures reproducibility, a critical factor in research and clinical settings.
ABPC recombinant proteins are widely utilized in therapeutics (e.g., vaccines, monoclonal antibodies), diagnostics (e.g., assay standards), and industrial enzymes. Their role in structural biology and drug discovery is pivotal, as they provide high-purity substrates for crystallography or inhibitor screening. Additionally, ABPC variants may serve as antigenic tools in antibody development or as functional replacements for deficient proteins in disease models.
Despite advantages like scalability and cost-effectiveness, challenges persist. These include ensuring proper folding in prokaryotic systems, minimizing host-induced contaminants, and addressing immunogenicity in therapeutic contexts. Ongoing advancements in expression optimization, cell-free systems, and AI-driven protein design aim to overcome these limitations, further expanding ABPC's utility.
In summary, ABPC recombinant protein represents a convergence of genetic engineering and biomanufacturing, offering versatile solutions across science and medicine. Its continued refinement underscores the dynamic evolution of recombinant technologies in addressing global health and industrial needs.
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