| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | CFX |
| Uniprot No | P24554 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-460aa |
| 氨基酸序列 | MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPMVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIEQICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL |
| 预测分子量 | 49,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于CFX重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
---
1. **文献名称**: *Efficient Expression and Purification of CFX Recombinant Protein in E. coli*
**作者**: Zhang, L.; Wang, H.; Chen, J.
**摘要**: 本研究报道了一种利用大肠杆菌表达系统高效表达CFX重组蛋白的方法，通过优化诱导条件和亲和层析技术，实现了高纯度蛋白的制备，并验证了其体外生物活性。
2. **文献名称**: *Structural and Functional Characterization of CFX: A Novel Recombinant Fusion Protein for Therapeutic Applications*
**作者**: Smith, R.; Johnson, A.
**摘要**: 作者解析了CFX重组融合蛋白的晶体结构，并通过细胞实验证明其具有抗炎和促修复功能，为开发基于CFX的靶向疗法提供了理论依据。
3. **文献名称**: *Scale-Up Production of CFX Recombinant Protein Using Mammalian Cell Culture System*
**作者**: Kim, S.; Lee, M.
**摘要**: 该研究建立了哺乳动物细胞(CHO细胞)大规模生产CFX重组蛋白的工艺，优化了培养基成分和生物反应器参数，显著提高了蛋白产量和糖基化修饰一致性。
4. **文献名称**: *CFX Recombinant Protein Enhances Wound Healing in Diabetic Mouse Models*
**作者**: Gupta, P.; Rao, K.
**摘要**: 实验证明，局部应用CFX重组蛋白可加速糖尿病小鼠皮肤创伤愈合，其机制可能与调控生长因子信号通路和减少氧化应激相关。
---
**建议**：若需真实文献，请通过以下方法检索：
1. 在 **PubMed** 或 **Google Scholar** 中搜索关键词：`"CFX recombinant protein"` 或 `"CFX protein expression"`。
2. 若“CFX”为特定蛋白缩写，需结合全称(如“Coagulation Factor X”)或相关领域缩小范围。
3. 查阅重组蛋白制备技术相关综述，追踪近期应用案例。
**Background of CFX Recombinant Protein**
CFX recombinant protein is a engineered biological molecule designed for diverse applications in biomedical research, therapeutic development, and industrial biotechnology. As a recombinant protein, it is produced through genetic engineering techniques, where the gene encoding the target protein is inserted into a host organism (e.g., *E. coli*, yeast, or mammalian cells) to enable large-scale production. The "CFX" designation typically refers to a specific protein variant or a proprietary formulation optimized for enhanced stability, solubility, or functional activity.
Recombinant proteins like CFX have revolutionized modern medicine by enabling precise control over protein structure and function. CFX may be derived from human or other species' protein templates, modified to eliminate immunogenicity or improve efficacy. Its development often involves codon optimization for efficient expression in the chosen host system, followed by purification via chromatography to ensure high purity.
In therapeutics, CFX recombinant protein could serve as a biologic drug targeting diseases such as cancer, autoimmune disorders, or infectious diseases. For instance, it might mimic cytokines, enzymes, or antibodies to modulate immune responses or replace deficient proteins. In research, it is widely used as a tool to study protein interactions, signaling pathways, or cellular mechanisms. Industrial applications include its use in biocatalysis or as a component in diagnostic assays.
The advantages of CFX recombinant protein include batch-to-batch consistency, scalability, and reduced reliance on animal-derived sources. Ongoing advancements in protein engineering, such as site-specific mutagenesis or fusion tags, continue to expand its functional versatility, making CFX a critical asset in both academic and commercial biotechnological endeavors.
×
