| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX6 |
| Uniprot No | P35712 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-745aa |
| 氨基酸序列 | MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA |
| 预测分子量 | 86,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SOX6重组蛋白的3篇代表性文献示例(注：内容基于研究领域常见方向整理，非真实文献，仅供参考)：
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1. **文献名称**: *SOX6 cooperates with SOX9 to promote cartilage-specific extracellular matrix remodeling*
**作者**: Kawasaki M, et al.
**摘要**: 研究通过重组SOX6蛋白与SOX9的共表达实验，揭示两者在软骨形成中的协同作用，调控胶原蛋白II和聚集多糖的表达，为骨骼发育机制提供依据。
2. **文献名称**: *Recombinant SOX6 protein inhibits β-cell proliferation by suppressing cyclin D2 transcription*
**作者**: Hargrave M, et al.
**摘要**: 利用大肠杆菌表达系统纯化SOX6重组蛋白，发现其通过直接结合DNA抑制胰岛β细胞周期蛋白D2的转录，提示其在糖尿病中的潜在调控作用。
3. **文献名称**: *Structural characterization of the SOX6 HMG domain and its DNA-binding specificity*
**作者**: Dy P, Lefebvre V.
**摘要**: 通过重组SOX6蛋白的HMG结构域结晶与体外DNA结合实验，解析其三维结构及靶向DNA序列偏好性，阐明其基因调控的分子基础。
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如需真实文献，建议在PubMed或Web of Science中检索关键词“recombinant SOX6 protein”或“SOX6 purification”。
**Background of SOX6 Recombinant Protein**
SOX6. a member of the SOX (Sry-related HMG box) family of transcription factors, plays critical roles in developmental processes, including neurogenesis, chondrogenesis, and cardiogenesis. It belongs to the SOXD group (SOX5. SOX6. SOX13) and functions by binding to DNA through its conserved HMG (High Mobility Group) domain, regulating gene expression in coordination with other transcription factors. SOX6 is particularly noted for its involvement in cell fate determination, differentiation, and tissue-specific regulatory networks.
Recombinant SOX6 protein is engineered *in vitro* using expression systems like *E. coli* or mammalian cells, enabling studies on its structure, function, and interactions. The protein is often purified via affinity tags (e.g., His-tag) and validated for activity through DNA-binding assays, Western blotting, or functional cellular assays. Its recombinant form is essential for elucidating mechanisms in developmental biology and disease contexts.
Research highlights SOX6's role in diseases such as cancer, where it may act as a tumor suppressor or oncogene depending on context, and in neurological disorders like autism or epilepsy. In cartilage development, SOX6 partners with SOX5 and SOX9 to drive chondrocyte differentiation. Dysregulation of SOX6 is also linked to metabolic syndromes and muscular disorders.
The availability of recombinant SOX6 accelerates drug discovery, CRISPR/Cas9-based functional studies, and therapeutic exploration for conditions tied to SOX6 dysfunction. Its study provides insights into transcriptional regulation and potential targets for regenerative medicine or precision therapies.
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