| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TEKT2 |
| Uniprot No | Q9UIF3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-430aa |
| 氨基酸序列 | MATLSVKPSR RFQLPDWHTN SYLLSTNAQL QRDASHQIRQ EARVLRNETN NQTIWDEHDN RTRLVERIDT VNRWKEMLDK CLTDLDAEID ALTQMKESAE QNLQAKNLPL DVAIECLTLR ESRRDIDVVK DPVEDELHKE VEVIEATKKA LQQKVSQAFE QLCLLQEVQQ QLNSDHRGKM ETLEIDRGCL SLNLRSPNIS LKVDPTRVPD GSTTLQQWDD FSRFNKDRAE AEMKAATELR EATALTIAET NNELEAQRVA TEFAFRKRLR EMEKVYSELK WQEKNTLEEI AELQEDIRHL EEDLRTKLLS LKLSHTRLEA RTYRPNVELC RDQAQYGLTD EVHQLEATIA ALKQKLAQAQ DALDALCKHL ARLQADIACK ANSMLLDTKC MDTRRKLTVP AERFVPEVDT FTRTTNSTLS PLKSCQLELA |
| 预测分子量 | 49,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TEKT2重组蛋白的3篇代表性参考文献(内容基于领域研究趋势模拟，具体文献需以实际数据库检索为准)：
1. **"Recombinant TEKT2 protein expression and its role in microtubule stability"**
- 作者：Li, X., et al. (2017)
- 摘要：该研究通过大肠杆菌系统成功表达并纯化重组TEKT2蛋白，发现其能够与微管蛋白结合并增强微管结构的稳定性，提示TEKT2可能在精子鞭毛形成或细胞分裂中发挥作用。
2. **"Structural characterization of human TEKT2 and its interaction with cytoskeletal components"**
- 作者：Wang, Y., et al. (2019)
- 摘要：通过圆二色谱和分子对接技术分析重组TEKT2蛋白的结构，揭示其螺旋卷曲区域对微管相关蛋白的结合能力，为研究TEKT2基因突变导致的不育症提供分子机制依据。
3. **"Functional analysis of TEKT2 mutations using recombinant protein in male infertility models"**
- 作者：Sato, K., et al. (2021)
- 摘要：构建携带TEKT2突变体的重组蛋白，发现部分突变导致蛋白二聚化能力丧失，并破坏精子运动相关微管网络的组装，支持TEKT2缺陷与男性不育的关联性。
**备注**：若需真实文献，建议通过PubMed或Google Scholar以“TEKT2 recombinant protein”“TEKT2 expression”等关键词检索近年发表的文章。部分研究可能因TEKT2在生殖系统中的特异性而集中在生殖医学领域。
TEKT2 (tektin 2) is a member of the tektin family of structural proteins that play critical roles in the assembly and stability of microtubule-based structures, particularly in cilia, flagella, and the sperm axoneme. These proteins are evolutionarily conserved and are essential for maintaining the integrity of microtubule networks in motile and sensory organelles. TEKT2 is predominantly expressed in the testes and is closely associated with sperm flagella formation, suggesting its importance in male fertility. Mutations or dysregulation of TEKT2 have been linked to structural abnormalities in sperm flagella, leading to reduced motility and infertility.
Recombinant TEKT2 protein is produced using biotechnological methods, such as expression in bacterial (e.g., *E. coli*) or eukaryotic systems, followed by purification via affinity chromatography. This engineered protein retains the functional domains of native TEKT2. enabling its use in studying microtubule dynamics, protein-protein interactions, and the molecular mechanisms underlying spermatogenesis. Researchers employ recombinant TEKT2 to investigate its role in cytoskeletal organization, cellular signaling, and its potential as a biomarker for diagnosing male reproductive disorders. Additionally, it serves as an antigen for antibody development or a tool for high-throughput screening in drug discovery aimed at addressing infertility. The study of TEKT2 and its recombinant form contributes to advancing reproductive biology and therapeutic strategies for related pathologies.
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