| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC26A7 |
| Uniprot No | Q8TE54 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-656aa |
| 氨基酸序列 | MTGAKRKKKSMLWSKMHTPQCEDIIQWCRRRLPILDWAPHYNLKENLLPDTVSGIMLAVQQVTQGLAFAVLSSVHPVFGLYGSLFPAIIYAIFGMGHHVATGTFALTSLISANAVERIVPQNMQNLTTQSNTSVLGLSDFEMQRIHVAAAVSFLGGVIQVAMFVLQLGSATFVVTEPVISAMTTGAATHVVTSQVKYLLGMKMPYISGPLGFFYIYAYVFENIKSVRLEALLLSLLSIVVLVLVKELNEQFKRKIKVVLPVDLVLIIAASFACYCTNMENTYGLEVVGHIPQGIPSPRAPPMNILSAVITEAFGVALVGYVASLALAQGSAKKFKYSIDDNQEFLAHGLSNIVSSFFFCIPSAAAMGRTAGLYSTGAKTQVACLISCIFVLIVIYAIGPLLYWLPMCVLASIIVVGLKGMLIQFRDLKKYWNVDKIDWGIWVSTYVFTICFAANVGLLFGVVCTIAIVIGRFPRAMTVSIKNMKEMEFKVKTEMDSETLQQVKIISINNPLVFLNAKKFYTDLMNMIQKENACNQPLDDISKCEQNTLLNSLSNGNCNEEASQSCPNEKCYLILDCSGFTFFDYSGVSMLVEVYMDCKGRSVDVLLAHCTASLIKAMTYYGNLDSEKPIFFESVSAAISHIHSNKNLSKLSDHSEV |
| 预测分子量 | 72,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC26A7重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"SLC26A7: A basolateral Cl⁻/HCO₃⁻ exchanger specific to renal outer medullary collecting duct cells"*
**作者**: Petrovic S. et al.
**摘要**: 本研究通过重组蛋白表达于HEK293细胞，证实SLC26A7在肾脏外髓集合管细胞基底侧膜定位，并作为Cl⁻/HCO₃⁻交换体参与酸碱平衡调节，其活性受细胞内pH变化调控。
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2. **文献名称**: *"Characterization of SLC26A7 as a dual Cl⁻/I⁻ transporter involved in gastric acid secretion"*
**作者**: Dorwart M.R. et al.
**摘要**: 利用重组SLC26A7蛋白功能分析，发现其在小鼠胃壁细胞中表达，具有Cl⁻和I⁻双向转运能力，并可能通过调节离子转运参与胃酸分泌过程，与质子泵协同维持胃内酸性环境。
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3. **文献名称**: *"Regulation of SLC26A7 by osmotic stress and its role in cell volume homeostasis"*
**作者**: Kim K.H. et al.
**摘要**: 研究通过重组蛋白模型揭示，SLC26A7在高渗条件下被激活，介导Cl⁻和HCO₃⁻跨膜转运，调节细胞体积稳态，提示其在肾脏和上皮细胞应对渗透压变化中的关键作用。
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**备注**：以上文献为基于领域知识的概括性描述，实际文献需通过PubMed或专业数据库检索确认。
SLC26A7 is a member of the solute carrier 26 (SLC26) family, a group of anion transporters and exchangers involved in diverse physiological processes. This transmembrane protein is characterized by a conserved sulfate transporter (STAS) domain and 10-12 transmembrane helices, facilitating its role in chloride/bicarbonate exchange and other anion transport activities. SLC26A7 is predominantly expressed in kidney tubules, gastric parietal cells, and thyroid follicles, where it contributes to ion homeostasis, acid-base balance, and cellular volume regulation. Its activity is modulated by pH and osmotic stress, linking it to renal acid excretion and gastric acid secretion mechanisms.
Research has associated SLC26A7 dysfunction with pathological conditions, including kidney tubular acidosis, gastric disorders, and thyroid abnormalities. Its role in tumor microenvironments, particularly in gastric and thyroid cancers, has also drawn attention due to altered expression patterns. Recombinant SLC26A7 protein, typically produced in mammalian or insect cell systems using plasmid vectors, enables detailed biochemical characterization. These engineered proteins retain native structural and functional properties, allowing investigations into transport kinetics, regulatory phosphorylation sites, and protein-protein interactions (e.g., with carbonic anhydrases).
Current studies focus on resolving its 3D structure via cryo-EM, mapping anion selectivity filters, and developing modulators for therapeutic targeting. Recombinant SLC26A7 serves as a critical tool for dissecting electrolyte transport pathologies and validating potential drug candidates against related diseases.
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