| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | P2RX5 |
| Uniprot No | Q93086 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-444aa |
| 氨基酸序列 | MGQAGCKGLCLSLFDYKTEKYVIAKNKKVGLLYRLLQASILAYLVVWVFLIKKGYQDVDTSLQSAVITKVKGVAFTNTSDLGQRIWDVADYVIPAQGENVFFVVTNLIVTPNQRQNVCAENEGIPDGACSKDSDCHAGEAVTAGNGVKTGRCLRRENLARGTCEIFAWCPLETSSRPEEPFLKEAEDFTIFIKNHIRFPKFNFSKSNVMDVKDRSFLKSCHFGPKNHYCPIFRLGSVIRWAGSDFQDIALEGGVIGINIEWNCDLDKAASECHPHYSFSRLDNKLSKSVSSGYNFRFARYYRDAAGVEFRTLMKAYGIRFDVMVNGKAGKFSIIPTIINVGSGVALMGAGAFFCDLVLIYLIKKREFYRDKKYEEVRGLEDSSQEAEDEASGLGLSEQLTSGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHRST |
| 预测分子量 | 49,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于P2RX5重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Structural and functional characterization of recombinant human P2X5 receptor*
**作者**: Garcia-Guzman, M., Soto, F., Gomez-Hernandez, J.M. et al.
**摘要**: 本研究通过哺乳动物细胞系统表达重组人源P2X5受体，分析了其ATP激活的离子通道特性。发现P2X5形成同源三聚体通道，在ATP刺激下介导钙离子内流，并揭示了其胞外结构域对配体结合的关键作用。
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2. **文献名称**: *P2X5 receptor regulates immune cell function through ATP-mediated signaling*
**作者**: Surprenant, A., Rassendren, F., Kawashima, E.
**摘要**: 通过重组P2X5蛋白实验，发现其在T淋巴细胞和树突状细胞中高表达。ATP激活P2X5后触发促炎细胞因子释放，并参与调控免疫细胞的增殖与凋亡，提示其在自身免疫疾病中的潜在作用。
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3. **文献名称**: *Cryo-EM structure of the ATP-gated P2X5 ion channel reveals a unique pore architecture*
**作者**: Kasuya, G., Fujiwara, Y., Nakane, T. et al.
**摘要**: 利用冷冻电镜解析重组小鼠P2X5受体的高分辨率结构，发现其不同于其他P2X家族成员的孔隙构象，并阐明了ATP结合后跨膜结构域的动态变构机制，为靶向药物设计提供结构基础。
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注：上述文献信息为示例，实际文献需通过数据库(如PubMed、Web of Science)检索确认。
**Background of P2RX5 Recombinant Protein**
P2RX5. a member of the P2X receptor family, is an ATP-gated ion channel involved in cellular signaling pathways. These receptors are trimeric proteins that respond to extracellular adenosine triphosphate (ATP), mediating cation flux (Na⁺, K⁺, Ca²⁺) across cell membranes. P2RX5 is expressed in immune cells (e.g., T cells, dendritic cells), osteoblasts, and certain epithelial tissues, playing roles in immune regulation, bone metabolism, and inflammation. Its activation triggers downstream processes like cytokine release, apoptosis, and cell differentiation.
Recombinant P2RX5 protein is engineered *in vitro* using heterologous expression systems (e.g., HEK293 cells, *E. coli*) to study its structure-function relationships, ligand interactions, and signaling mechanisms. The recombinant form allows researchers to bypass challenges associated with native protein isolation, ensuring high purity and yield. Studies using this tool have clarified P2RX5’s role in autoimmune diseases (e.g., rheumatoid arthritis) and bone disorders, as well as its paradoxical functions in cancer progression—acting as either a tumor suppressor or promoter depending on context.
Recent structural studies, leveraging recombinant P2RX5. have revealed details about its ATP-binding site and ion-conducting pathways, aiding drug discovery efforts. Inhibitors or modulators targeting P2RX5 are explored for therapies against osteoporosis, chronic inflammation, and malignancies. However, its broad tissue distribution and complex signaling crosstalk necessitate careful *in vivo* validation. Overall, recombinant P2RX5 remains a critical tool for decoding purinergic signaling and developing targeted therapeutics.
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