| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXT2 |
| Uniprot No | Q93063 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-718aa |
| 氨基酸序列 | MCASVKYNIRGPALIPRMKTKHRIYYITLFSIVLLGLIATGMFQFWPHSIESSNDWNVEKRSIRDVPVVRLPADSPIPERGDLSCRMHTCFDVYRCGFNPKNKIKVYIYALKKYVDDFGVSVSNTISREYNELLMAISDSDYYTDDINRACLFVPSIDVLNQNTLRIKETAQAMAQLSRWDRGTNHLLFNMLPGGPPDYNTALDVPRDRALLAGGGFSTWTYRQGYDVSIPVYSPLSAEVDLPEKGPGPRQYFLLSSQVGLHPEYREDLEALQVKHGESVLVLDKCTNLSEGVLSVRKRCHKHQVFDYPQVLQEATFCVVLRGARLGQAVLSDVLQAGCVPVVIADSYILPFSEVLDWKRASVVVPEEKMSDVYSILQSIPQRQIEEMQRQARWFWEAYFQSIKAIALATLQIINDRIYPYAAISYEEWNDPPAVKWGSVSNPLFLPLIPPQSQGFTAIVLTYDRVESLFRVITEVSKVPSLSKLLVVWNNQNKNPPEDSLWPKIRVPLKVVRTAENKLSNRFFPYDEIETEAVLAIDDDIIMLTSDELQFGYEVWREFPDRLVGYPGRLHLWDHEMNKWKYESEWTNEVSMVLTGAAFYHKYFNYLYTYKMPGDIKNWVDAHMNCEDIAMNFLVANVTGKAVIKVTPRKKFKCPECTAIDGLSLDQTHMVERSECINKFASVFGTMPLKVVEHRADPVLYKDDFPEKLKSFPNIGSL |
| 预测分子量 | 82,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EXT2重组蛋白的3篇参考文献示例(注：以下内容为模拟文献，实际引用请核对真实论文)：
1. **文献名称**: *"Expression and purification of recombinant EXT2 protein in E. coli for functional studies"*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组EXT2蛋白，通过亲和层析纯化后验证其活性。实验表明，重组EXT2能够与EXT1形成复合物，催化硫酸乙酰肝素链的延伸。
2. **文献名称**: *"Structural insights into the EXT2 protein: Implications for hereditary multiple exostoses"*
**作者**: Zhang L, et al.
**摘要**: 通过X射线晶体学解析了人源重组EXT2蛋白的晶体结构，揭示了其糖基转移酶活性关键位点，为遗传性多发性骨软骨瘤的致病突变机制提供了分子基础。
3. **文献名称**: *"Functional characterization of EXT2 mutations using recombinant protein assays"*
**作者**: Tanaka K, et al.
**摘要**: 构建了多种EXT2突变体重组蛋白，通过体外酶活实验证实特定突变导致糖基转移功能丧失，解释了患者表型与基因型的关联性。
如需具体文献，建议通过PubMed或Google Scholar搜索关键词：**EXT2 recombinant protein, EXT1/EXT2 complex, heparan sulfate biosynthesis**。
The EXT2 (Exostosin Glycosyltransferase 2) recombinant protein is derived from the EXT2 gene, which encodes a critical enzyme involved in heparan sulfate (HS) biosynthesis. EXT2. along with its homolog EXT1. forms a hetero-oligomeric complex localized in the Golgi apparatus. This complex functions as a glycosyltransferase, catalyzing the elongation of heparan sulfate chains—a type of glycosaminoglycan (GAG) essential for cell signaling, extracellular matrix interactions, and molecular trafficking. HS proteoglycans (HSPGs) play pivotal roles in developmental processes, angiogenesis, and cellular communication.
Mutations in the EXT2 gene are associated with hereditary multiple exostoses (HME), an autosomal dominant disorder characterized by benign bone tumors (osteochondromas). Studying EXT2 recombinant protein helps elucidate its structural and functional properties, including its interaction with EXT1 and its role in HS chain polymerization. Recombinant EXT2 is typically produced using expression systems like mammalian cells or bacteria, enabling in vitro analysis of enzymatic activity, substrate specificity, and potential therapeutic interventions.
Research on EXT2 also extends to cancer biology, as altered HS expression correlates with tumor progression and metastasis. The recombinant protein serves as a tool for investigating HS-dependent pathways, such as fibroblast growth factor (FGF) signaling, and for developing targeted therapies. Additionally, it aids in diagnosing HME and understanding genotype-phenotype relationships. Overall, EXT2 recombinant protein is vital for advancing both basic science and clinical applications related to HS metabolism and associated disorders.
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