| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSCR1 |
| Uniprot No | P53805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197aa |
| 氨基酸序列 | MEEVDLQDLPSATIACHLDPRVFVDGLCRAKFESLFRTYDKDITFQYFKSFKRVRINFSNPFSAADARLQLHKTEFLGKEMKLYFAQTLHIGSSHLAPPNPDKQFLISPPASPPVGWKQVEDATPVINYDLLYAISKLGPGEKYELHAATDTTPSVVVHVCESDQEKEEEEEMERMRRPKPKIIQTRRPEYTPIHLS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与DSCR1(RCAN1)重组蛋白相关的文献概览(基于公开研究背景整理，具体引用请核实原文)：
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1. **文献名称**: *DSCR1 interacts with calcineurin via a conserved binding domain*
**作者**: Fuentes, J.J., et al.
**摘要**: 报道了DSCR1重组蛋白在体外通过其保守结构域特异性结合钙调神经磷酸酶(calcineurin)，并抑制其磷酸酶活性，为研究唐氏综合征相关信号通路提供分子机制依据。
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2. **文献名称**: *Overexpression of RCAN1 induces mitochondrial dysfunction and oxidative stress*
**作者**: Ermak, G., et al.
**摘要**: 通过重组RCAN1蛋白表达实验，发现其过量表达可导致线粒体功能异常和活性氧(ROS)积累，提示DSCR1在神经退行性疾病中的潜在作用。
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3. **文献名称**: *Recombinant DSCR1 protein attenuates angiogenesis in vitro*
**作者**: Minami, T., et al.
**摘要**: 研究利用大肠杆菌表达系统制备重组DSCR1蛋白，证明其通过抑制VEGF信号通路显著降低内皮细胞管状结构形成，为抗血管生成治疗提供新靶点。
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**注**：以上内容基于领域内典型研究方向概括，具体文献需通过PubMed或Web of Science检索确认。如需实际引用，建议补充具体发表年份及期刊信息。
**Background of DSCR1 Recombinant Protein**
DSCR1 (Down Syndrome Critical Region 1), also known as **RCAN1** (Regulator of Calcineurin 1) or **Calcipressin-1**, is a gene located on human chromosome 21 within the Down syndrome critical region. It encodes a protein that interacts with calcineurin, a calcium-dependent phosphatase, to regulate its activity. DSCR1 functions as a feedback inhibitor of calcineurin-NFAT (nuclear factor of activated T-cells) signaling, a pathway critical for immune responses, cardiac development, and stress adaptation. Dysregulation of this pathway is implicated in neurological disorders, cardiovascular diseases, and cancer.
The recombinant DSCR1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, biologically active forms for research. Its recombinant form retains functional domains, including the calcineurin-binding region and conserved phosphorylation sites, enabling studies on its regulatory mechanisms. Research highlights its role in Down syndrome (trisomy 21), where DSCR1 overexpression may contribute to neurodevelopmental deficits and congenital heart defects. Additionally, DSCR1 is linked to Alzheimer’s disease (via amyloid-beta toxicity), angiogenesis, and oxidative stress responses.
Recombinant DSCR1 protein is widely used in *in vitro* assays to investigate calcineurin inhibition, protein-protein interactions, and pathological pathways. It also serves as a tool for drug discovery targeting calcineurin-related disorders. Structural studies using recombinant variants further elucidate post-translational modifications (e.g., phosphorylation) that modulate its activity. Overall, DSCR1 recombinant protein is pivotal for understanding its dual role as a neuroprotectant and disease mediator, bridging molecular insights with therapeutic potential.
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