| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHb3 |
| Uniprot No | Q9Y5E6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-796aa |
| 氨基酸序列 | MEAGGERFLRQRQVLLLFVFLGGSLAGSESRRYSVAEEKEKGFLIANLAKDLGLRVEELAARGAQVVSKGNKQHFQLSHQTGDLLLNEKLDREELCGPTEPCILHFQILLQNPLQFVTNELRIIDVNDHSPVFFENEMHLKILESTLPGTVIPLGNAEDLDVGRNSLQNYTITPNSHFHVLTRSRRDGRKYPELVLDKALDPEEQPELSLTLTALDGGSPPRSGTAQINIQVLDINDNAPEFAQPLYEVAVLENTPVNSVIVTVSASDLDTGSFGTISYAFFHASEEIRKTFQLNPITGDMQLVKYLNFEAINSYEVDIEAKDGGGLSGKSTVIVQVVDVNDNPPELTLSSVNSPIPENSGETVLAVFSVSDLDSGDNGRVMCSIENNLPFFLKPSVENFYTLVSEGALDRETRSEYNITITITDLGTPRLKTKYNITVLVSDVNDNAPAFTQISYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSLLPPQDPHLPLSSLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPALSSEALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKHRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADLLTVYLVVALASVSSLFLFSVLLFVAVRLCRRSRAASVGRCSVPEGPFPGQMVDVSGTGTLSQSYQYEVCLTGGSGTNEFKFLKPIIPNFVAQGAERVSEANPSFRKSFEFS |
| 预测分子量 | 86,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHb3重组蛋白的3篇参考文献示例(文献信息为假设性描述，实际需根据具体论文补充)：
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1. **文献名称**: "Recombinant PCDHb3 promotes neuronal adhesion through homophilic interactions"
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组PCDHb3蛋白，证实其通过同源亲和机制介导神经元细胞间的特异性粘附，为神经回路形成机制提供了新证据。
2. **文献名称**: "Structural analysis of PCDHb3 recombinant protein reveals its role in cancer cell signaling"
**作者**: Zhang Y, et al.
**摘要**: 通过哺乳动物细胞系统表达PCDHb3重组蛋白，解析其胞外结构域与表皮生长因子受体的相互作用，揭示其在肿瘤细胞迁移中的潜在调控功能。
3. **文献名称**: "PCDHb3 knockout and recombinant rescue experiments implicate its function in synaptic plasticity"
**作者**: Lee JH, et al.
**摘要**: 利用重组PCDHb3蛋白恢复基因敲除模型的突触功能，证明其对海马神经元突触可塑性的关键作用，可能与认知障碍类疾病相关。
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**注**：以上为模拟内容，实际文献需通过PubMed、Web of Science等平台以关键词“PCDHb3 recombinant”或“Protocadherin beta 3 recombinant protein”检索。若研究较少，可扩展检索原钙粘蛋白(Protocadherin)家族或相关信号通路文献。
**Background of PCDHb3 Recombinant Protein**
Protocadherin beta-3 (PCDHb3) is a member of the protocadherin β gene cluster, a subgroup within the cadherin superfamily of calcium-dependent cell adhesion molecules. These transmembrane proteins play critical roles in neural development, including axon guidance, synaptic specificity, and neuronal circuit formation. The protocadherin β cluster, located on human chromosome 5. consists of 16 genes (PCDHb1 to PCDHb16) that encode diverse isoforms. PCDHb3 is particularly notable for its involvement in establishing cell-cell recognition and adhesion through homophilic interactions, which are essential for maintaining neural connectivity and tissue architecture.
Recombinant PCDHb3 protein is engineered *in vitro* by expressing the PCDHb3 gene in heterologous systems, such as mammalian cells (e.g., HEK293) or bacterial systems, followed by purification. This protein typically retains key functional domains, including extracellular cadherin repeats and a conserved cytoplasmic domain, enabling studies on its structural and mechanistic properties. Researchers utilize recombinant PCDHb3 to investigate its role in neurodevelopment, synaptic plasticity, and potential links to neurological disorders. For instance, dysregulation of protocadherins has been implicated in autism spectrum disorders (ASD), schizophrenia, and epilepsy, making PCDHb3 a target for exploring disease mechanisms.
Furthermore, recombinant PCDHb3 serves as a tool for developing antibodies, mapping interaction partners, and screening therapeutic compounds. Its applications extend to *in vitro* assays, such as cell adhesion or neurite outgrowth experiments, and *in vivo* models to validate functional pathways. Recent studies also highlight its involvement in cancer progression, particularly in metastasis and cell signaling, broadening its relevance beyond neuroscience. By providing a controlled, high-purity form of the protein, recombinant PCDHb3 accelerates both basic research and translational efforts to uncover its biological significance and therapeutic potential.
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