| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP1a4 |
| Uniprot No | Q13733 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1029aa |
| 氨基酸序列 | MGLWGKKGTVAPHDQSPRRRPKKGLIKKKMVKREKQKRNMEELKKEVVMDDHKLTLEELSTKYSVDLTKGHSHQRAKEILTRGGPNTVTPPPTTPEWVKFCKQLFGGFSLLLWTGAILCFVAYSIQIYFNEEPTKDNLYLSIVLSVVVIVTGCFSYYQEAKSSKIMESFKNMVPQQALVIRGGEKMQINVQEVVLGDLVEIKGGDRVPADLRLISAQGCKVDNSSLTGESEPQSRSPDFTHENPLETRNICFFSTNCVEGTARGIVIATGDSTVMGRIASLTSGLAVGQTPIAAEIEHFIHLITVVAVFLGVTFFALSLLLGYGWLEAIIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDMTVYEADTTEEQTGKTFTKSSDTWFMLARIAGLCNRADFKANQEILPIAKRATTGDASESALLKFIEQSYSSVAEMREKNPKVAEIPFNSTNKYQMSIHLREDSSQTHVLMMKGAPERILEFCSTFLLNGQEYSMNDEMKEAFQNAYLELGGLGERVLGFCFLNLPSSFSKGFPFNTDEINFPMDNLCFVGLISMIDPPRAAVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGTETAEEVAARLKIPISKVDASAAKAIVVHGAELKDIQSKQLDQILQNHPEIVFARTSPQQKLIIVEGCQRLGAVVAVTGDGVNDSPALKKADIGIAMGISGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIMYTLTSNIPEITPFLMFIILGIPLPLGTITILCIDLGTDMVPAISLAYESAESDIMKRLPRNPKTDNLVNHRLIGMAYGQIGMIQALAGFFTYFVILAENGFRPVDLLGIRLHWEDKYLNDLEDSYGQQWTYEQRKVVEFTCQTAFFVTIVVVQWADLIISKTRRNSLFQQGMRNKVLIFGILEETLLAAFLSYTPGMDVALRMYPLKITWWLCAIPYSILIFVYDEIRKLLIRQHPDGWVERETYY |
| 预测分子量 | 114 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP1a4重组蛋白的3篇模拟参考文献(非真实文献，仅供示例参考)：
1. **文献名称**: "Functional characterization of recombinant ATP1a4 in HEK293 cells: Role in sodium-potassium transport"
**作者**: Smith JL, et al.
**摘要**: 本研究通过将ATP1a4基因克隆至HEK293细胞中进行重组表达，证实其编码的钠钾ATP酶α4亚型具有独特的离子转运活性。实验表明，ATP1a4对低浓度钾离子的亲和力显著高于其他α亚型，提示其在特定生理环境(如雄性生殖系统)中的功能重要性。
2. **文献名称**: "ATP1a4 knockout and recombinant rescue models reveal its essential role in sperm motility"
**作者**: Tanaka K, et al.
**摘要**: 通过构建ATP1a4基因敲除小鼠，发现其精子活力显著下降。进一步利用重组ATP1a4蛋白体外补充实验，证实该蛋白通过调节精子细胞内的钠钾离子梯度，维持鞭毛运动的能量供应，为男性不育机制研究提供了新方向。
3. **文献名称**: "Crystallographic analysis of ATP1a4 recombinant protein reveals isoform-specific structural motifs"
**作者**: Zhang R, et al.
**摘要**: 本研究首次解析了重组ATP1a4蛋白的晶体结构，揭示了其跨膜结构域中特有的氨基酸残基排列。与α1亚型相比，ATP1a4的胞内ATP结合口袋更狭窄，可能影响其与特定抑制剂的相互作用，为开发靶向药物提供了结构基础。
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注：以上文献为示例性内容，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。
ATP1a4. also known as the α4 subunit of Na+/K+-ATPase, is a critical ion transport protein responsible for maintaining electrochemical gradients across cell membranes. As a member of the P-type ATPase family, it catalyzes ATP hydrolysis to actively transport three Na⁺ ions out of the cell and two K⁺ ions into the cell per cycle. This enzyme consists of α and β subunits, with the α subunit (like ATP1a4) forming the catalytic core. ATP1a4 is one of four α-isoforms (α1–α4) in mammals, distinguished by tissue-specific expression and functional properties. While α1 is ubiquitously expressed, ATP1a4 is predominantly found in spermatozoa, specific neurons, and certain endocrine tissues, suggesting specialized roles in cellular excitability, motility, or hormone secretion.
Recombinant ATP1a4 protein is engineered using heterologous expression systems (e.g., insect or mammalian cells) to study its structure, regulation, and interactions. Its production enables detailed biochemical analyses, including ATPase activity assays, ion transport kinetics, and inhibitor sensitivity profiling. ATP1a4 has garnered interest due to its unique physiological and pathological implications. For instance, it contributes to sperm hyperactivation during fertilization and may influence neuronal signaling in brain regions like the hippocampus. Dysregulation of ATP1a4 has been linked to male infertility and neurological disorders, while its sensitivity to cardiac glycosides (e.g., ouabain) highlights potential therapeutic targets.
Research on recombinant ATP1a4 aids in elucidating isoform-specific functions of Na+/K+-ATPases, advancing drug development for related diseases. Its study also provides insights into evolutionary adaptations, as certain species exhibit α4 variants with distinct ion affinities. Overall, ATP1a4 recombinant protein serves as a vital tool for exploring ion homeostasis mechanisms and their implications in health and disease.
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