关键信息
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基因名
CD40L/CD154/TRAP
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简介
CD40L/CD154/TRAP proteins stimulate T cell proliferation and cytokine production, acting as ligands for integrins and CD40 receptors to activate various cell-dependent effects such as B cell activation and NF-κ-B signaling. CD40L/CD154/TRAP Protein, Human (HEK293, mFc) is the recombinant human-derived CD40L/CD154/TRAP protein, expressed by HEK293, with N-mFc labeled tag.
- 应用
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别名
rHuCD40L; CD154; TRAP; TNFSF5; CD40LG; CD40-L
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种属
Human
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表达系统
HEK293
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标签
N-mFc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P29965
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表达区间
H47-L261
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蛋白长度
Extracellular Domain
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分子量
45-50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CD40L, also known as CD154, is a crucial membrane protein primarily expressed on activated T cells and plays a pivotal role in immune system regulation by providing essential signals to B cells and other antigen-presenting cells through CD40 receptor engagement. This interaction is vital for processes such as B cell activation, proliferation, and isotype switching, all of which are fundamental for generating effective adaptive immune responses. In addition, CD40L is implicated in various immunological disorders, including autoimmune diseases and cancers, making it an attractive target for therapeutic interventions. To study its functions and potential applications, researchers have developed recombinant CD40L proteins, often referred to as TRAP (trimeric association protein), which can mimic the natural signaling of CD40L. These recombinant proteins facilitate the investigation of CD40/CD40L pathways in controlled settings, enabling the exploration of their roles in immune responses and paving the way for innovative treatments that harness this pathway. Understanding the structure and function of CD40L/TRAP enhances our knowledge of immune mechanisms and could lead to novel strategies for modulating immune responses in various diseases.
