| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYOF |
| Uniprot No | Q9NZM1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-2061aa |
| 氨基酸序列 | MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV |
| 预测分子量 | 234 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYOF(Myoferlin)重组蛋白研究的虚构参考文献示例(基于学术文献常见结构模拟，非真实存在)：
1. **Title**: Structural and functional characterization of recombinant human MYOF in membrane repair mechanisms
**Authors**: Chen L, Wang X, Rodriguez M, et al.
**Journal**: Journal of Biological Chemistry, 2020. 295(12): 4012-4025.
**摘要**: 本研究通过杆状病毒表达系统重组表达人源MYOF蛋白，解析其C2结构域与磷脂膜的相互作用机制，证实其在细胞膜修复过程中介导囊泡融合的关键功能。
2. **Title**: MYOF recombinant protein suppresses tumor metastasis via EGFR signaling modulation
**Authors**: Kim S, Tanaka R, Gupta P.
**Journal**: Cell Reports, 2018. 24(5): 1289-1301.
**摘要**: 利用哺乳动物细胞表达系统制备功能性MYOF重组蛋白，证明其通过调控EGFR内吞作用抑制乳腺癌细胞迁移，为癌症治疗提供新靶点。
3. **Title**: High-yield production of bioactive MYOF in E. coli: Purification and in vitro validation
**Authors**: Müller J*, Schmidt A, Wagner K.
**Journal**: Protein Expression and Purification, 2019. 158: 30-38.
**摘要**: 首次报道大肠杆菌中可溶性MYOF重组蛋白的包涵体复性策略，通过离子交换层析获得高纯度蛋白，并验证其在体外模型中的肌管形成促进作用。
4. **Title**: MYOF-mediated exosome biogenesis requires calcium-dependent protein oligomerization
**Authors**: Santos C, Li H (co-first author), O'Neil D.
**Journal**: Scientific Reports, 2021. 11: 23045.
**摘要**: 采用His标签重组MYOF蛋白进行pull-down实验，揭示其依赖Ca²⁰形成多聚体调控外泌体生成的分子机制，为肌肉疾病研究提供新方向。
注：以上文献信息为模拟创作，实际研究中请通过PubMed/Web of Science等平台检索真实文献。
**Background of MYOF Recombinant Protein**
Myoferlin (MYOF), a member of the ferlin protein family, is a calcium-regulated membrane-associated protein implicated in cell membrane repair, vesicle trafficking, and cellular signaling. Structurally, it contains multiple C2 domains that mediate lipid binding and membrane interactions, along with a conserved FerA domain critical for its function. MYOF is highly expressed in tissues with high membrane turnover, such as skeletal muscle, endothelial cells, and certain cancer cells, where it supports processes like fusion of intracellular vesicles, repair of plasma membrane injuries, and regulation of receptor tyrosine kinase signaling.
Recombinant MYOF protein is engineered using expression systems (e.g., mammalian, bacterial, or insect cells) to produce purified, functional MYOF for research and therapeutic applications. Its production enables studies on MYOF’s role in diseases like muscular dystrophy, where membrane repair is compromised, and in cancer, where MYOF overexpression correlates with enhanced metastasis, chemoresistance, and proliferation. Researchers utilize recombinant MYOF to investigate its interactions with partners like dysferlin or EGFR, screen for inhibitors, or develop antibody-based therapies.
Challenges in MYOF recombinant protein production include preserving post-translational modifications (e.g., phosphorylation) and ensuring proper folding for functional assays. Current efforts focus on optimizing expression systems and purification protocols to enhance yield and stability. As a potential therapeutic target, MYOF’s involvement in pathological angiogenesis and tumor progression underscores its biomedical relevance, driving interest in recombinant tools to unravel its mechanisms and therapeutic potential.
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