| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BEST3 |
| Uniprot No | Q8N1M1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-668aa |
| 氨基酸序列 | MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGVQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKHGHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMFLPRDDLSPARDLLDVPSRNPPRASPTWKKSCFPEGSPTLHFSMGELSTIRETSQTSTLQSLTPQSSVRTSPIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPSPQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIVAGSRVSSDMLYLMENLDTKETDIIELNKETEESPK |
| 预测分子量 | 76,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BEST3重组蛋白的假设性参考文献示例(建议通过学术数据库验证具体信息)：
---
1. **"Functional characterization of recombinant human BEST3 chloride channels in HEK293 cells"**
*Smith J, et al. (2018)*
摘要：研究通过重组表达人源BEST3蛋白于HEK293细胞，证实其氯离子通道功能，并分析其电生理特性及调控机制。
2. **"Structural insights into BEST3 protein by cryo-EM: Implications for ion transport"**
*Zhang L, et al. (2020)*
摘要：利用冷冻电镜解析重组BEST3蛋白的高分辨率结构，揭示其离子选择性及与疾病相关突变的结构基础。
3. **"BEST3 knockout and rescue model reveals its role in retinal homeostasis"**
*Johnson R, et al. (2021)*
摘要：通过基因编辑构建BEST3敲除细胞模型，重组BEST3蛋白回补实验证明其对视网膜细胞钙离子调控的关键作用。
4. **"Optimization of recombinant BEST3 expression and purification for drug screening"**
*Lee S, et al. (2019)*
摘要：开发高效重组BEST3蛋白的大肠杆菌表达系统及纯化策略，用于靶向药物筛选及功能抑制剂研究。
---
**注意**：以上为假设文献，建议通过PubMed、Google Scholar等平台检索真实文献(关键词：BEST3 recombinant protein/ion channel)。实际研究中可关注以下方向：
- BEST3与视网膜病变的分子机制
- 重组蛋白的离子通道功能验证
- 结构生物学研究及疾病相关突变分析
**Background of BEST3 Recombinant Protein**
The BEST3 (Bestrophin-3) protein, encoded by the *BEST3* gene in humans, belongs to the bestrophin family of anion channels, which regulate chloride (Cl⁻) and bicarbonate (HCO₃⁻) transport across cell membranes. BEST3 is primarily expressed in epithelial and smooth muscle tissues, playing roles in ion homeostasis, cell volume regulation, and intracellular signaling. Structurally, it contains four transmembrane domains, cytoplasmic N- and C-termini, and a conserved "aperture domain" critical for ion conduction.
BEST3 gained attention due to its association with genetic disorders. Mutations in *BEST3* are linked to autosomal dominant bestrophinopathy, a retinal disease affecting retinal pigment epithelium function. However, recent studies suggest broader implications, including potential roles in cancer progression, cardiovascular function, and gastrointestinal motility. For instance, BEST3 is implicated in gastric acid secretion and smooth muscle contraction, highlighting its physiological versatility.
As a recombinant protein, BEST3 is produced via heterologous expression systems (e.g., *E. coli*, mammalian cells) for functional studies. Recombinant BEST3 enables detailed analysis of its ion channel properties, structure-function relationships, and interactions with disease-associated mutants. It also serves as a tool for drug screening to identify modulators of anion transport, which may have therapeutic potential for BEST3-related disorders.
Research on BEST3 recombinant protein continues to expand, bridging gaps in understanding its biological significance and translational applications in ion channelopathies and beyond.
×
