| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMD8 |
| Uniprot No | P48556 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-350aa |
| 氨基酸序列 | MFIKGRAPRA PPRERRRATR GGLRQVVAPP RALGSTSRPH FRRASVCRRR CRKSGGLLAA SRKMAAAAVN GAAGFSSSGP AATSGAVLQA ATGMYEQLKG EWNRKSPNLS KCGEELGRLK LVLLELNFLP TTGTKLTKQQ LILARDILEI GAQWSILRKD IPSFERYMAQ LKCYYFDYKE QLPESAYMHQ LLGLNLLFLL SQNRVAEFHT ELERLPAKDI QTNVYIKHPV SLEQYLMEGS YNKVFLAKGN IPAESYTFFI DILLDTIRDE IAGCIEKAYE KILFTEATRI LFFNTPKKMT DYAKKRGWVL GPNNYYSFAS QQQKPEDTTI PSTELAKQVI EYARQLEMIV |
| 预测分子量 | 39,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMD8重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant expression and functional characterization of the 26S proteasome subunit PSMD8"*
**作者**: Tanaka K, et al.
**摘要**: 本研究成功在大肠杆菌中重组表达了PSMD8蛋白，并验证其与蛋白酶体其他亚基的相互作用。实验表明，PSMD8对26S蛋白酶体的组装和底物识别具有关键作用，并揭示了其C端结构域在结合泛素链中的重要性。
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2. **文献名称**: *"Structural insights into the regulatory particle of the proteasome: Analysis of PSMD8 through X-ray crystallography"*
**作者**: Smith J, et al.
**摘要**: 通过X射线晶体学解析了重组PSMD8蛋白的高分辨率结构，发现其具有独特的α螺旋结构域，可能参与稳定蛋白酶体调节颗粒的构象。研究为靶向PSMD8的抑制剂设计提供了结构基础。
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3. **文献名称**: *"PSMD8 knockdown impairs tumor cell proliferation via dysregulation of the ubiquitin-proteasome system"*
**作者**: Li X, et al.
**摘要**: 利用重组PSMD8蛋白进行功能研究，发现其缺失导致癌细胞中异常蛋白积累并诱导凋亡。研究证实PSMD8在肿瘤中高表达，可能成为癌症治疗的潜在靶点。
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**注**：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science核对具体信息。如需扩展，可搜索关键词“PSMD8 recombinant expression”或“proteasome regulatory particle assembly”获取更多研究。
**Background of PSMD8 Recombinant Protein**
PSMD8 (Proteasome 26S Subunit, Non-ATPase 8) is a critical component of the 26S proteasome, a large multi-subunit complex responsible for targeted protein degradation in eukaryotic cells. As part of the 19S regulatory particle, PSMD8 plays a key role in the ubiquitin-proteasome system (UPS), which regulates cellular processes like cell cycle progression, DNA repair, and stress responses by degrading ubiquitin-tagged proteins. The 19S subunit recognizes polyubiquitin signals, unfolds substrates, and translocates them into the 20S catalytic core for proteolysis.
Structurally, PSMD8 is a non-ATPase subunit that stabilizes the 19S regulatory complex and facilitates interactions between other proteasomal components. Dysregulation of PSMD8 has been linked to pathologies such as cancer, neurodegenerative diseases, and autoimmune disorders, highlighting its importance in maintaining proteostasis.
Recombinant PSMD8 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional forms for *in vitro* studies. It enables researchers to investigate PSMD8’s molecular interactions, structural dynamics, and role in proteasome assembly/activity. Additionally, it serves as a tool for screening therapeutic agents targeting the UPS, particularly in cancers where proteasome inhibitors (e.g., bortezomib) are clinically utilized. Studies leveraging recombinant PSMD8 also aim to unravel its potential as a biomarker or drug target in diseases associated with impaired protein degradation.
Overall, PSMD8 recombinant protein is vital for advancing mechanistic insights into proteasome function and developing novel UPS-targeted therapies.
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