| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUSP6 |
| Uniprot No | Q16828 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MIDTLRPVPF ASEMAISKTV AWLNEQLELG NERLLLMDCR PQELYESSHI ESAINVAIPG IMLRRLQKGN LPVRALFTRG EDRDRFTRRC GTDTVVLYDE SSSDWNENTG GESVLGLLLK KLKDEGCRAF YLEGGFSKFQ AEFSLHCETN LDGSCSSSSP PLPVLGLGGL RISSDSSSDI ESDLDRDPNS ATDSDGSPLS NSQPSFPVEI LPFLYLGCAK DSTNLDVLEE FGIKYILNVT PNLPNLFENA GEFKYKQIPI SDHWSQNLSQ FFPEAISFID EARGKNCGVL VHCLAGISRS VTVTVAYLMQ KLNLSMNDAY DIVKMKKSNI SPNFNFMGQL LDFERTLGLS SPCDNRVPAQ QLYFTTPSNQ NVYQVDSLQS T |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DUSP6重组蛋白的3篇参考文献示例(注：以下内容为模拟生成的示例，实际文献需通过学术数据库检索确认)：
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1. **标题**: "Recombinant DUSP6 protein inhibits ERK signaling and suppresses pancreatic cancer cell proliferation"
**作者**: Zhang Y, et al.
**摘要**: 研究通过大肠杆菌表达系统纯化重组人DUSP6蛋白，证实其可特异性去磷酸化ERK1/2.抑制胰腺癌细胞增殖并诱导凋亡，为靶向治疗提供依据。
2. **标题**: "Crystal structure of human DUSP6 reveals a regulatory mechanism for substrate specificity"
**作者**: Jeong DG, et al.
**摘要**: 解析了重组DUSP6蛋白的晶体结构，揭示了其活性位点与底物ERK的结合模式，并发现N端结构域通过变构调节催化活性，为设计选择性抑制剂奠定基础。
3. **标题**: "DUSP6 recombinant protein ameliorates cardiac hypertrophy via modulating MAPK pathway in diabetic rats"
**作者**: Wang L, et al.
**摘要**: 在糖尿病大鼠模型中，外源性重组DUSP6蛋白通过抑制病理性心肌肥厚相关的ERK过度激活，改善心脏功能，提示其治疗代谢性心血管疾病的潜力。
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建议通过PubMed或Google Scholar以关键词“DUSP6 recombinant protein”或“DUSP6 purification”检索最新文献，重点关注其重组表达方法、功能验证或疾病相关机制研究。
DUSP6 (Dual Specificity Phosphatase 6), also known as MKP-3. is a member of the dual-specificity phosphatase family that regulates mitogen-activated protein kinase (MAPK) signaling pathways. It specifically dephosphorylates and inactivates extracellular signal-regulated kinase 1/2 (ERK1/2), a critical component of the RAS/RAF/MEK/ERK cascade involved in cell proliferation, differentiation, and stress responses. DUSP6 acts as a negative feedback regulator of ERK activity, ensuring precise control of signaling duration and intensity. Its expression is often induced by ERK-dependent transcription factors, creating a self-limiting loop to prevent hyperactivation of the pathway.
Structurally, DUSP6 contains a conserved phosphatase domain and a kinase interaction motif (KIM) that mediates binding to ERK. Unlike other MAPK phosphatases, DUSP6 exhibits cytoplasmic localization due to its lack of nuclear localization signals. It is highly expressed in tissues such as the heart, pancreas, and central nervous system, where ERK signaling plays vital roles in development and homeostasis.
Dysregulation of DUSP6 has been implicated in multiple diseases. Reduced expression or functional mutations are linked to cancers (e.g., pancreatic, glioblastoma), diabetes, and cardiovascular disorders, often correlating with uncontrolled ERK activation. Conversely, overexpression may contribute to neurodegenerative conditions. Recombinant DUSP6 protein, typically produced in *E. coli* or mammalian expression systems with affinity tags (e.g., His-tag), serves as a tool to study ERK pathway modulation, screen therapeutic compounds, or explore gene therapy strategies. Its therapeutic potential lies in restoring balanced MAPK signaling, making it a target for diseases driven by ERK dysregulation.
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