| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUSP19 |
| Uniprot No | Q8WTR2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-217aa |
| 氨基酸序列 | MYSLNQEIKA FSRNNLRKQC TRVTTLTGKK IIETWKDARI HVVEEVEPSS GGGCGYVQDL SSDLQVGVIK PWLLLGSQDA AHDLDTLKKN KVTHILNVAY GVENAFLSDF TYKSISILDL PETNILSYFP ECFEFIEEAK RKDGVVLVHC NAGVSRAAAI VIGFLMNSEQ TSFTSAFSLV KNARPSICPN SGFMEQLRTY QEGKESNKCD RIQENSS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DUSP19重组蛋白的示例参考文献(仅供参考，非真实文献)：
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1. **"Cloning and Functional Characterization of DUSP19 in MAPK Signaling Pathway"**
*Author: Zhang et al. (2018)*
**摘要**：研究报道了DUSP19重组蛋白在大肠杆菌中的高效表达及纯化，证实其通过去磷酸化p38和JNK蛋白负调控MAPK信号通路，并在炎症反应中发挥潜在作用。
2. **"Structural Insights into DUSP19 Phosphatase Activity and Substrate Specificity"**
*Author: Lee & Kim (2020)*
**摘要**：通过X射线晶体学解析DUSP19重组蛋白的三维结构，揭示其催化域的关键氨基酸残基对底物选择性的影响，并证明其对ERK1/2的弱活性。
3. **"DUSP19 Recombinant Protein Attenuates Cardiac Hypertrophy via Modulating AKT/mTOR Pathway"**
*Author: Wang et al. (2021)*
**摘要**：在心肌细胞模型中，重组DUSP19蛋白通过抑制AKT/mTOR信号通路减轻病理性心脏肥大，提示其作为治疗靶点的潜力。
4. **"Development of a High-Yield Mammalian Expression System for DUSP19 and Its Role in Apoptosis Regulation"**
*Author: Gupta et al. (2019)*
**摘要**：利用HEK293细胞实现DUSP19重组蛋白的高效分泌表达，并证明其通过调控Bcl-2家族蛋白抑制TNFα诱导的细胞凋亡。
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*注：以上文献为示例性质，具体研究请通过PubMed或Web of Science等数据库检索真实文献。*
DUSP19 (Dual Specificity Phosphatase 19) is a member of the dual-specificity phosphatase family, which regulates mitogen-activated protein kinase (MAPK) signaling pathways by dephosphorylating both phosphotyrosine and phosphoserine/phosphothreonine residues on target proteins. It is also known as low-molecular-mass DUSP or SKRP1 (stress-activated protein kinase pathway-regulating phosphatase 1). DUSP19 is primarily expressed in immune tissues, such as the spleen and thymus, and plays roles in cellular stress responses, immune regulation, and inflammation. Its enzymatic activity helps maintain MAPK signaling homeostasis, impacting processes like cell proliferation, differentiation, and apoptosis.
Recombinant DUSP19 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional protein for research. This tool enables studies on DUSP19’s structure, substrate specificity, and interaction with MAPKs (e.g., JNK, p38). Researchers use it to explore its regulatory mechanisms in diseases, including cancer, autoimmune disorders, and neurodegenerative conditions. For example, DUSP19’s involvement in modulating inflammatory cytokines links it to pathologies like rheumatoid arthritis. Additionally, recombinant DUSP19 aids in drug discovery, serving as a target for inhibitors or activators to fine-tune MAPK pathways therapeutically. Challenges in its study include understanding tissue-specific roles and post-translational modifications affecting activity. Current research focuses on clarifying its physiological relevance and translational potential in precision medicine.
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