| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUSP18 |
| Uniprot No | Q8NEJ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-188aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMTAPSCAFPV QFRQPSVSGL SQITKSLYIS NGVAANNKLM LSSNQITMVI NVSVEVVNTL YEDIQYMQVP VADSPNSRLC DFFDPIADHI HSVEMKQGRT LLHCAAGVSR SAALCLAYLM KYHAMSLLDA HTWTKSCRPI IRPNSGFWEQ LIHYEFQLFG KNTVHMVSSP VGMIPDIYEK EVRLMIPL |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的3篇关于DUSP18重组蛋白的参考文献示例。请注意，这些文献信息为假设性内容，建议通过学术数据库(如PubMed、Web of Science)查询真实文献：
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1. **标题**: *"Recombinant Expression and Enzymatic Characterization of Dual Specificity Phosphatase DUSP18"*
**作者**: Zhang Y. et al.
**摘要**: 本研究在大肠杆菌系统中成功表达并纯化了人源DUSP18重组蛋白，验证其对p38 MAPK和JNK的特异性去磷酸化活性。结果显示，DUSP18在酸性pH条件下活性最高，为研究其生理功能提供了体外酶学基础。
2. **标题**: *"DUSP18 Modulates Inflammatory Responses in Macrophages via Regulation of STAT1 Signaling"*
**作者**: Li H. et al.
**摘要**: 通过重组DUSP18蛋白的细胞递送实验，发现其通过抑制STAT1磷酸化降低LPS诱导的巨噬细胞炎症因子(如TNF-α、IL-6)分泌，提示DUSP18可能在先天免疫中起负调控作用。
3. **标题**: *"Structural Insights into the Catalytic Mechanism of DUSP18 by X-ray Crystallography"*
**作者**: Chen X. et al.
**摘要**: 本研究解析了重组DUSP18蛋白的晶体结构(分辨率2.1Å)，揭示了其活性位点中保守的Cys残基与底物结合的关键构象，为设计靶向DUSP18的小分子抑制剂提供了结构基础。
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如需进一步研究，建议结合具体研究方向(如疾病模型、信号通路)在学术数据库中检索真实文献。
DUSP18 (Dual Specificity Phosphatase 18) is a member of the dual-specificity phosphatase family, which regulates mitogen-activated protein kinase (MAPK) signaling pathways by dephosphorylating both tyrosine and serine/threonine residues. MAPKs, including ERK, JNK, and p38. are critical for cellular processes such as proliferation, differentiation, and apoptosis. DUSP18 acts as a negative regulator of these pathways, fine-tuning their activity to maintain cellular homeostasis. Although less studied compared to other DUSP family members (e.g., DUSP1 or DUSP6), DUSP18 is implicated in immune regulation, metabolic processes, and stress responses, with potential roles in inflammation, cancer, and metabolic disorders.
Recombinant DUSP18 protein is produced using genetic engineering techniques, often expressed in bacterial or mammalian systems to ensure proper folding and enzymatic activity. Its production enables functional studies, including substrate specificity assays, interaction analyses with MAPKs, and exploration of its regulatory mechanisms. Structural studies of recombinant DUSP18 may reveal conserved catalytic domains (e.g., the phosphatase domain) and unique motifs that differentiate it from other DUSPs. Researchers also utilize this protein to investigate its tissue-specific expression patterns, with reports suggesting elevated levels in liver, kidney, and immune cells.
Current research focuses on DUSP18’s therapeutic potential. For instance, its dysregulation has been linked to inflammatory diseases and cancer progression, making it a candidate for drug targeting. Additionally, recombinant DUSP18 serves as a tool for screening small-molecule inhibitors or activators to modulate MAPK pathways. Challenges include understanding its substrate preferences in vivo and clarifying its context-dependent roles in different pathological conditions. Further studies are needed to unravel its full biological significance and translational applications.
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