| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYNLRB2 |
| Uniprot No | Q8TF09 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-96aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAEVEETLKRIQSHKGVIGTMVVNAEGIPI RTTLDNSTTVQYAGLLHHLTMKAKSTVRDIDPQNDLTFLRIRSKKHEIMV APDKEYLLIVIQNPCE |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DYNLRB2重组蛋白的参考文献示例(注：以下内容为模拟概括，具体文献需通过学术数据库核实)：
1. **文献名称**：*Structural insights into the dynein light chain DYNLRB2 and its interaction with the viral protein*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析DYNLRB2重组蛋白的三维结构，揭示其与某病毒蛋白的结合位点，探讨其在病毒感染中的潜在作用机制。
2. **文献名称**：*DYNLRB2 regulates mitotic spindle orientation via binding to NuMA in cancer cells*
**作者**：Wang Y, et al.
**摘要**：研究重组DYNLRB2蛋白与核有丝分裂器蛋白(NuMA)的相互作用，发现其通过调控纺锤体方向影响肿瘤细胞的增殖和迁移。
3. **文献名称**：*Expression and purification of recombinant human DYNLRB2 in E. coli for functional studies*
**作者**：Kim S, et al.
**摘要**：优化大肠杆菌中DYNLRB2重组蛋白的可溶性表达和纯化方案，验证其与动力蛋白复合体的结合活性，为后续功能研究提供基础。
4. **文献名称**：*DYNLRB2 dysfunction disrupts lysosomal trafficking and promotes neurodegenerative pathology*
**作者**：Zhang R, et al.
**摘要**：利用重组DYNLRB2蛋白进行体外实验，发现其缺失导致溶酶体运输异常，可能与阿尔茨海默病中的神经元退行性变相关。
建议通过PubMed、Web of Science等平台以“DYNLRB2 recombinant protein”或“DYNLRB2 structure/function”为关键词检索最新文献。
DYNLRB2 (Dynein Light Chain Roadblock-Type 2) is a component of the cytoplasmic dynein motor complex, which plays a critical role in intracellular transport along microtubules. This protein belongs to the roadblock/LC7 family of dynein light chains, known for their involvement in regulating dynein-mediated cargo trafficking, organelle positioning, and cell division. DYNLRB2 specifically interacts with the dynein complex to modulate its function, acting as a molecular adaptor that links dynein to specific cargoes or regulatory proteins. Structurally, it forms a homodimer and binds to partner proteins through conserved hydrophobic grooves, facilitating the assembly of functional dynein motor units.
Research highlights DYNLRB2's role in neuronal function, where it contributes to axonal transport, synaptic vesicle trafficking, and maintenance of cellular polarity. Dysregulation of DYNLRB2 has been implicated in neurodegenerative disorders, such as Alzheimer’s disease, and certain cancers, where altered dynein activity disrupts mitotic processes or intracellular signaling pathways. Recombinant DYNLRB2 proteins are engineered using expression systems like *E. coli* or mammalian cells, enabling studies on its structure, binding partners, and mechanistic contributions to dynein regulation. These proteins are often tagged (e.g., His, GST) for purification and experimental applications, including co-immunoprecipitation, *in vitro* binding assays, and structural analysis. By leveraging recombinant DYNLRB2. researchers aim to decipher its role in cellular dynamics and explore therapeutic strategies targeting dynein-associated pathologies.
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