Recombinant Human PTPRT protein

**Background of PTPRT Recombinant Protein**

Protein tyrosine phosphatase receptor type T (PTPRT), also known as PTPrho, is a member of the protein tyrosine phosphatase (PTP) superfamily, which plays critical roles in regulating cellular signaling pathways by dephosphorylating tyrosine residues on target proteins. PTPRT is a transmembrane receptor-type phosphatase characterized by an extracellular fibronectin III domain, a single transmembrane region, and an intracellular catalytic domain. It is broadly expressed in various tissues, including the brain, kidney, and colon, and has been implicated in cell adhesion, growth, and differentiation.

Research highlights PTPRT’s role as a tumor suppressor, particularly in colorectal and lung cancers. Somatic mutations in the *PTPRT* gene are frequently observed in human cancers, often leading to loss of phosphatase activity and dysregulation of signaling pathways, such as STAT3. which promotes tumor progression. Recombinant PTPRT protein, produced via expression systems like mammalian (e.g., HEK293) or insect cells (e.g., Sf9), retains enzymatic activity and structural integrity, enabling functional studies. It is widely used to investigate substrate specificity, inhibition mechanisms, and interactions with signaling molecules.

The recombinant protein also serves as a tool for developing therapeutic strategies targeting PTPRT-deficient cancers. Studies explore its potential in restoring normal phosphatase activity or modulating downstream oncogenic pathways. Despite progress, challenges remain in understanding its full spectrum of substrates and regulatory networks. Overall, PTPRT recombinant protein is a vital resource for dissecting its biological roles and translational applications in cancer and beyond.