| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | mpt64 |
| Uniprot No | P9WIN9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-228aa |
| 氨基酸序列 | APKTYCEELKGTDTGQACQIQMSDPAYNINISLPSYYPDQKSLENYIAQTRDKFLSAATSSTPREAPYELNITSATYQSAIPPRGTQAVVLKVYQNAGGTHPTTTYKAFDWDQAYRKPITYDTLWQADTDPLPVVFPIVQGELSKQTGQQVSIAPNAGLDPVNYQNFAVTNDGVIFFFNPGELLPEAAGPTQVLVPRSAIDSMLA |
| 预测分子量 | 23.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MPT64重组蛋白的3篇参考文献示例(文献标题和作者为虚构示例，供参考)：
1. **文献名称**: *Cloning and expression of Mycobacterium tuberculosis MPT64 recombinant protein in Escherichia coli*
**作者**: Smith A, et al.
**摘要**: 该研究通过克隆MPT64基因(Rv1985c)至大肠杆菌表达系统，成功表达并纯化重组MPT64蛋白。通过Western blot验证其抗原性，表明其可用于结核病血清学诊断。
2. **文献名称**: *Diagnostic potential of recombinant MPT64 for tuberculosis detection in clinical samples*
**作者**: Lee B, et al.
**摘要**: 评估重组MPT64蛋白在结核病诊断中的敏感性及特异性，结果表明其与患者血清抗体结合率高，可作为快速检测试剂的候选抗原。
3. **文献名称**: *Immunogenicity analysis of MPT64 as a subunit vaccine candidate against tuberculosis*
**作者**: Zhang C, et al.
**摘要**: 研究重组MPT64蛋白在小鼠模型中的免疫应答，发现其可诱导Th1型细胞免疫和特异性抗体产生，提示其作为结核亚单位疫苗的潜力。
如需真实文献，建议通过PubMed或Google Scholar检索关键词“MPT64 recombinant protein”或“Rv1985c expression”获取具体文章。
**Background of MPT64 Recombinant Protein**
MPT64 (also known as Rv1980c) is a secreted protein produced by *Mycobacterium tuberculosis* (Mtb), the causative agent of tuberculosis (TB). It is a 24 kDa antigenic protein encoded by the *mpt64* gene and is part of the mycobacterial secretome during active infection. MPT64 is recognized for its immunogenic properties, playing a role in host-pathogen interactions and modulating immune responses. Structurally, it contains a conserved domain characteristic of the PE/PPE protein family, though it lacks the typical Pro-Glu (PE) or Pro-Pro-Glu (PPE) motifs. Instead, MPT64 features a β-barrel fold, which may facilitate its interaction with host cells or extracellular components.
Recombinant MPT64 is produced via genetic engineering, often expressed in heterologous systems like *Escherichia coli* to ensure high purity and yield for research and diagnostic applications. Its recombinant form retains antigenic epitopes, making it valuable in serological assays to detect TB-specific antibodies in patient sera. Additionally, MPT64 is utilized in interferon-gamma release assays (IGRAs) to evaluate cell-mediated immune responses, aiding in distinguishing latent TB infection from active disease.
Studies suggest MPT64 contributes to Mtb virulence by suppressing host immune defenses, though its exact mechanism remains under investigation. Notably, some clinical Mtb strains exhibit mutations in the *mpt64* gene, leading to truncated or nonfunctional protein expression, which complicates diagnostic reliability. This variability underscores the importance of recombinant MPT64 as a standardized reagent in TB research and diagnostics.
Overall, MPT64 recombinant protein serves as a critical tool for understanding TB pathogenesis, developing diagnostics, and evaluating vaccine candidates, highlighting its dual role as a biomarker and a functional mediator in mycobacterial infections.
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