| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EBNA1BP2 |
| Uniprot No | Q99848 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-306aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMDTPPLS DSESESDESL VTDRELQDAF SRGLLKPGLN VVLEGPKKAV NDVNGLKQCL AEFKRDLEWV ERLDVTLGPV PEIGGSEAPA PQNKDQKAVD PEDDFQREMS FYRQAQAAVL AVLPRLHQLK VPTKRPTDYF AEMAKSDLQM QKIRQKLQTK QAAMERSEKA KQLRALRKYG KKVQTEVLQK RQQEKAHMMN AIKKYQKGFS DKLDFLEGDQ KPLAQRKKAG AKGQQMRKGP SAKRRYKNQK FGFGGKKKGS KWNTRESYDD VSSFRAKTAH GRGLKRPGKK GSNKRPGKRT REKMKNRTH |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EBNA1BP2重组蛋白的3篇参考文献及其摘要概述：
1. **文献名称**：*"EBNA1BP2 interacts with nucleolar ribosomal proteins and is involved in ribosomal RNA processing"*
**作者**：Hirano, M., et al. (2005)
**摘要**：该研究通过重组EBNA1BP2蛋白实验，揭示其作为核仁定位蛋白与核糖体亚基蛋白的相互作用，参与rRNA前体的加工和核糖体生物合成。
2. **文献名称**：*"Functional characterization of recombinant EBNA1BP2 in cell cycle regulation"*
**作者**：Yuan, X., et al. (2006)
**摘要**：利用重组EBNA1BP2蛋白进行功能分析，发现其通过调控p53通路影响细胞周期G1/S期转换，提示其在肿瘤发生中的潜在作用。
3. **文献名称**：*"Structural insights into the RNA-binding domain of EBNA1BP2 using recombinant protein crystallography"*
**作者**：Choudhury, P., et al. (2018)
**摘要**：通过重组蛋白结晶技术解析EBNA1BP2的RNA结合域三维结构，阐明其与28S rRNA特定序列结合的分子机制。
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**备注**：上述文献为示例性质，实际研究中建议通过PubMed或Web of Science等平台以关键词“EBNA1BP2 recombinant”或“EBP2 protein purification”检索最新文献。
EBNA1BP2 (EBNA1-binding protein 2), also known as EBP2. is a conserved nucleolar protein implicated in ribosome biogenesis and chromatin interactions. Initially identified through its interaction with Epstein-Barr virus nuclear antigen 1 (EBNA1), it plays a role in EBV episome maintenance during viral latency. Beyond viral biology, EBNA1BP2 is essential in eukaryotic cells for rRNA processing and ribosome assembly. It localizes to the nucleolus and associates with ribosomal DNA (rDNA), facilitating 28S rRNA maturation and coordinating steps in ribosome subunit formation. Structural studies reveal its association with nucleolar proteins like PeBoW complex components, suggesting a scaffolding role in large ribonucleoprotein complexes.
Recombinant EBNA1BP2 proteins are commonly expressed in bacterial (e.g., E. coli) or eukaryotic systems to study molecular interactions and functional domains. These tools enable investigation of its binding affinity for EBNA1. which involves specific acidic regions critical for viral genome tethering to host chromosomes. Research using purified EBNA1BP2 has clarified its dual role: supporting viral persistence through EBNA1 interaction while regulating cellular growth via ribosome synthesis. Dysregulation of EBNA1BP2 correlates with ribosomopathies and cancers, highlighting its importance in cell proliferation. Current studies focus on its structural motifs, post-translational modifications, and potential as a therapeutic target in EBV-associated malignancies. Recombinant protein approaches remain pivotal for dissecting its roles in both viral pathogenesis and fundamental nucleolar functions.
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